Stimulation of the T3-T cell receptor complex induces a membrane-potential-sensitive calcium influx.

Published

Journal Article

Three monoclonal antibodies selected for their recognition of parts of the T3-T cell receptor complex on human T lymphocytes were found to induce an increase in cytoplasmic free Ca2+ (Ca2+i) in the T cell leukemia line HPB-ALL as measured by Quin2 fluorescence. These reagents are directed against T3 (OKT3), a nonvariable T3-associated structure (WT-31) and the variable region of the T3-associated antigen receptor (T40/25) of this cell line. The rise in Ca2+i was dependent on the presence of extracellular Ca2+, occurred within 30 sec of stimulation, and was sustained for at least 10 min. Fab fragments of OKT3 also caused a rapid increase in Ca2+i, indicating that cross-linking is not necessary to induce a Ca2+ response. Alterations in plasma membrane potential and La3+ blocked the Ca2+ influx induced by OKT3 and T40/25. These data suggest that the T3-T cell receptor complex of human T lymphocytes may be an antigen-regulated Ca2+ channel.

Full Text

Duke Authors

Cited Authors

  • Oettgen, HC; Terhorst, C; Cantley, LC; Rosoff, PM

Published Date

  • March 1, 1985

Published In

Volume / Issue

  • 40 / 3

Start / End Page

  • 583 - 590

PubMed ID

  • 2578888

Pubmed Central ID

  • 2578888

International Standard Serial Number (ISSN)

  • 0092-8674

Language

  • eng

Conference Location

  • United States