Antibody valence and induced signal transduction: the role of antibody valence in anti-CD3-induced signal transduction in isolated normal T cells.

Journal Article (Journal Article)

This report provides direct evidence that protein kinase C (PKC) is activated in isolated, rigorously accessory cell (AC)-depleted T cells when the T cell antigen recognition complex is stimulated by divalent anti-CD3 monoclonal antibody. Anti-CD3 monoclonal antibody-stimulated PKC activation alone does not, however, directly stimulate T cell proliferation in the absence of AC. A rise in cytosolic calcium is the second signal believed to be of paramount importance in T cell activation. While mitogenic concentrations of some divalent anti-CD3 antibodies do not cause a rise in cytosolic calcium, polyvalent anti-CD3 does evoke increased intracellular Ca2+ in rigorously AC-depleted resting human T cells.

Full Text

Duke Authors

Cited Authors

  • Melton, LB; Lakkis, F; Moscovitch-Lopatin, M; Smith, BR; Williams, JM; Rosoff, PM; Strom, TB

Published Date

  • February 1989

Published In

Volume / Issue

  • 50 / 2

Start / End Page

  • 171 - 183

PubMed ID

  • 2521583

International Standard Serial Number (ISSN)

  • 0090-1229

Digital Object Identifier (DOI)

  • 10.1016/0090-1229(89)90126-8


  • eng

Conference Location

  • United States