Essential role of LAT in T cell development.


Journal Article

The linker molecule LAT is a substrate of the tyrosine kinases activated following TCR engagement. Phosphorylated LAT binds many critical signaling molecules. The central role of this molecule in TCR-mediated signaling has been demonstrated by experiments in a LAT-deficient cell line. To probe the role of LAT in T cell development, the LAT gene was disrupted by targeting. LAT-deficient mice appeared healthy. Flow cytometric analysis revealed normal B cell populations but the absence of any mature peripheral T cells. Intrathymic development was blocked within the CD4- CD8- stage. No gross abnormality of NK or platelet function was observed. LAT is thus critical to both T cell activation and development.

Full Text

Duke Authors

Cited Authors

  • Zhang, W; Sommers, CL; Burshtyn, DN; Stebbins, CC; DeJarnette, JB; Trible, RP; Grinberg, A; Tsay, HC; Jacobs, HM; Kessler, CM; Long, EO; Love, PE; Samelson, LE

Published Date

  • March 1999

Published In

Volume / Issue

  • 10 / 3

Start / End Page

  • 323 - 332

PubMed ID

  • 10204488

Pubmed Central ID

  • 10204488

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/s1074-7613(00)80032-1


  • eng

Conference Location

  • United States