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Reduced stress defense in heme oxygenase 1-deficient cells.

Publication ,  Journal Article
Poss, KD; Tonegawa, S
Published in: Proc Natl Acad Sci U S A
September 30, 1997
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Stressed mammalian cells up-regulate heme oxygenase 1 (Hmox1; EC 1.14.99.3), which catabolizes heme to biliverdin, carbon monoxide, and free iron. To assess the potential role of Hmox1 in cellular antioxidant defense, we analyzed the responses of cells from mice lacking functional Hmox1 to oxidative challenges. Cultured Hmox1(-/-) embryonic fibroblasts demonstrated high oxygen free radical production when exposed to hemin, hydrogen peroxide, paraquat, or cadmium chloride, and they were hypersensitive to cytotoxicity caused by hemin and hydrogen peroxide. Furthermore, young adult Hmox1(-/-) mice were vulnerable to mortality and hepatic necrosis when challenged with endotoxin. Our in vitro and in vivo results provide genetic evidence that up-regulation of Hmox1 serves as an adaptive mechanism to protect cells from oxidative damage during stress.

Duke Scholars

Kenneth Daniel Poss
Author Kenneth Daniel Poss Cell Biology
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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.94.20.10925

ISSN

0027-8424

Publication Date

September 30, 1997

Volume

94

Issue

20

Start / End Page

10925 / 10930

Location

United States

Related Subject Headings

  • Oxidative Stress
  • Mice
  • Male
  • Lipopolysaccharides
  • Iron
  • Homeostasis
  • Heme Oxygenase (Decyclizing)
  • Female
  • Endotoxemia
  • Animals
 

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Poss, K. D., & Tonegawa, S. (1997). Reduced stress defense in heme oxygenase 1-deficient cells. Proc Natl Acad Sci U S A, 94(20), 10925–10930. https://doi.org/10.1073/pnas.94.20.10925
Poss, K. D., and S. Tonegawa. “Reduced stress defense in heme oxygenase 1-deficient cells.” Proc Natl Acad Sci U S A 94, no. 20 (September 30, 1997): 10925–30. https://doi.org/10.1073/pnas.94.20.10925.
Poss KD, Tonegawa S. Reduced stress defense in heme oxygenase 1-deficient cells. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10925–30.
Poss, K. D., and S. Tonegawa. “Reduced stress defense in heme oxygenase 1-deficient cells.” Proc Natl Acad Sci U S A, vol. 94, no. 20, Sept. 1997, pp. 10925–30. Pubmed, doi:10.1073/pnas.94.20.10925.
Poss KD, Tonegawa S. Reduced stress defense in heme oxygenase 1-deficient cells. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10925–10930.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.94.20.10925

ISSN

0027-8424

Publication Date

September 30, 1997

Volume

94

Issue

20

Start / End Page

10925 / 10930

Location

United States

Related Subject Headings

  • Oxidative Stress
  • Mice
  • Male
  • Lipopolysaccharides
  • Iron
  • Homeostasis
  • Heme Oxygenase (Decyclizing)
  • Female
  • Endotoxemia
  • Animals