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Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy.

Publication ,  Journal Article
Kirkman, MS; Simel, DL; Rao, KM; Hatchell, DL
Published in: Am J Med Sci
February 1994

Capillary closure in diabetic retinopathy may be initiated by lumenal occlusion by granulocytes. To determine whether subjects with diabetes mellitus have less deformable granulocytes than healthy subjects, granulocyte deformability was measured by mean entry time into a model capillary system in 16 diabetic-nondiabetic pairs. Granulocyte F-(filamentous) actin content between groups was compared, under basal conditions and after cellular stimulation. The relationship of granulocyte deformability to several diabetes-related variables was examined. Diabetic granulocytes were only 9% +/- 22% less deformable than normal granulocytes (p = 0.16). Deformability was increased in subjects with retinopathy and those with the worst glycemic control (r = 0.61, p = 0.026); both findings were in the opposite direction from that predicted. Basal and stimulated granulocyte F-actin content did not differ between the two groups (p > 0.2 for all assays). Although granulocytes may be important in the pathogenesis of diabetic retinopathy, granulocyte deformability (measured by mean entry time) and F-actin content are not significantly different between healthy patients and those with diabetes.

Duke Scholars

Published In

Am J Med Sci

DOI

ISSN

0002-9629

Publication Date

February 1994

Volume

307

Issue

2

Start / End Page

86 / 91

Location

United States

Related Subject Headings

  • Reference Values
  • N-Formylmethionine Leucyl-Phenylalanine
  • Middle Aged
  • Male
  • Humans
  • Granulocytes
  • Glycated Hemoglobin
  • General & Internal Medicine
  • Female
  • Diabetic Retinopathy
 

Citation

APA
Chicago
ICMJE
MLA
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Kirkman, M. S., Simel, D. L., Rao, K. M., & Hatchell, D. L. (1994). Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy. Am J Med Sci, 307(2), 86–91. https://doi.org/10.1097/00000441-199402000-00003
Kirkman, M. S., D. L. Simel, K. M. Rao, and D. L. Hatchell. “Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy.Am J Med Sci 307, no. 2 (February 1994): 86–91. https://doi.org/10.1097/00000441-199402000-00003.
Kirkman MS, Simel DL, Rao KM, Hatchell DL. Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy. Am J Med Sci. 1994 Feb;307(2):86–91.
Kirkman, M. S., et al. “Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy.Am J Med Sci, vol. 307, no. 2, Feb. 1994, pp. 86–91. Pubmed, doi:10.1097/00000441-199402000-00003.
Kirkman MS, Simel DL, Rao KM, Hatchell DL. Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy. Am J Med Sci. 1994 Feb;307(2):86–91.
Journal cover image

Published In

Am J Med Sci

DOI

ISSN

0002-9629

Publication Date

February 1994

Volume

307

Issue

2

Start / End Page

86 / 91

Location

United States

Related Subject Headings

  • Reference Values
  • N-Formylmethionine Leucyl-Phenylalanine
  • Middle Aged
  • Male
  • Humans
  • Granulocytes
  • Glycated Hemoglobin
  • General & Internal Medicine
  • Female
  • Diabetic Retinopathy