Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy.
Published
Journal Article
Capillary closure in diabetic retinopathy may be initiated by lumenal occlusion by granulocytes. To determine whether subjects with diabetes mellitus have less deformable granulocytes than healthy subjects, granulocyte deformability was measured by mean entry time into a model capillary system in 16 diabetic-nondiabetic pairs. Granulocyte F-(filamentous) actin content between groups was compared, under basal conditions and after cellular stimulation. The relationship of granulocyte deformability to several diabetes-related variables was examined. Diabetic granulocytes were only 9% +/- 22% less deformable than normal granulocytes (p = 0.16). Deformability was increased in subjects with retinopathy and those with the worst glycemic control (r = 0.61, p = 0.026); both findings were in the opposite direction from that predicted. Basal and stimulated granulocyte F-actin content did not differ between the two groups (p > 0.2 for all assays). Although granulocytes may be important in the pathogenesis of diabetic retinopathy, granulocyte deformability (measured by mean entry time) and F-actin content are not significantly different between healthy patients and those with diabetes.
Full Text
Duke Authors
Cited Authors
- Kirkman, MS; Simel, DL; Rao, KM; Hatchell, DL
Published Date
- February 1994
Published In
Volume / Issue
- 307 / 2
Start / End Page
- 86 - 91
PubMed ID
- 8141143
Pubmed Central ID
- 8141143
International Standard Serial Number (ISSN)
- 0002-9629
Digital Object Identifier (DOI)
- 10.1097/00000441-199402000-00003
Language
- eng
Conference Location
- United States