Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages.

Journal Article (Journal Article)

Corynebacterium parvum-activated macrophages (M phi), purified by adherence, were cytotoxic for B16 melanoma cells maintained in vitro. Pretreatment of the melanoma cells for 18 hr with interferon-alpha/beta or -gamma (IFN-alpha/beta or -gamma) caused a reduced susceptibility of the B16 cells to M phi-mediated cytotoxicity. The IFN-induced protective effect of B16 cells from cytotoxic M phi was found to be dose dependent. In addition, IFN-gamma was more protective than IFN-alpha/beta. The protective effect observed with partially purified IFN was reproduced by using highly purified IFN-alpha/beta or recombinant IFN-gamma. Monoclonal antibodies to IFN-gamma neutralized the protective effect provided by IFN-gamma. These results show that the susceptibility of a tumor cell line to killing by activated M phi can be altered by IFN pretreatment.

Full Text

Duke Authors

Cited Authors

  • Sarzotti, M; Baron, S; Tyring, SK; Klimpel, GR

Published Date

  • June 1986

Published In

Volume / Issue

  • 100 / 1

Start / End Page

  • 280 - 287

PubMed ID

  • 2427205

International Standard Serial Number (ISSN)

  • 0008-8749

Digital Object Identifier (DOI)

  • 10.1016/0008-8749(86)90028-6


  • eng

Conference Location

  • Netherlands