Scholars@Duke publication: Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages.

Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages.

Publication , Journal Article

Sarzotti, M; Baron, S; Tyring, SK; Klimpel, GR

Published in: Cell Immunol

June 1986

Corynebacterium parvum-activated macrophages (M phi), purified by adherence, were cytotoxic for B16 melanoma cells maintained in vitro. Pretreatment of the melanoma cells for 18 hr with interferon-alpha/beta or -gamma (IFN-alpha/beta or -gamma) caused a reduced susceptibility of the B16 cells to M phi-mediated cytotoxicity. The IFN-induced protective effect of B16 cells from cytotoxic M phi was found to be dose dependent. In addition, IFN-gamma was more protective than IFN-alpha/beta. The protective effect observed with partially purified IFN was reproduced by using highly purified IFN-alpha/beta or recombinant IFN-gamma. Monoclonal antibodies to IFN-gamma neutralized the protective effect provided by IFN-gamma. These results show that the susceptibility of a tumor cell line to killing by activated M phi can be altered by IFN pretreatment.

Duke Scholars

Author Marcella Sarzotti-Kelsoe Integrative Immunobiology

Published In

Cell Immunol

DOI

10.1016/0008-8749(86)90028-6

ISSN

0008-8749

Publication Date

June 1986

Volume

100

Issue

Start / End Page

280 / 287

Location

Netherlands

Related Subject Headings

Recombinant Proteins
Propionibacterium acnes
Mice
Melanoma
Macrophages
Macrophage Activation
Interferons
Immunology
Immunity, Cellular
Cytotoxicity, Immunologic

Citation

APA

Chicago

ICMJE

MLA

NLM

Sarzotti, M., Baron, S., Tyring, S. K., & Klimpel, G. R. (1986). Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages. Cell Immunol, 100(1), 280–287. https://doi.org/10.1016/0008-8749(86)90028-6

Sarzotti, M., S. Baron, S. K. Tyring, and G. R. Klimpel. “Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages.” Cell Immunol 100, no. 1 (June 1986): 280–87. https://doi.org/10.1016/0008-8749(86)90028-6.

Sarzotti M, Baron S, Tyring SK, Klimpel GR. Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages. Cell Immunol. 1986 Jun;100(1):280–7.

Sarzotti, M., et al. “Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages.” Cell Immunol, vol. 100, no. 1, June 1986, pp. 280–87. Pubmed, doi:10.1016/0008-8749(86)90028-6.

Sarzotti M, Baron S, Tyring SK, Klimpel GR. Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages. Cell Immunol. 1986 Jun;100(1):280–287.