Determining nuclear morphology using an improved angle-resolved low coherence interferometry system.

Published

Journal Article

We outline the process for determining the morphology of subsurface epithelial cell nuclei using depth-resolved light scattering measurements. The measurements are accomplished using a second generation angle-resolved low coherence interferometry system. The new system greatly improves data acquisition and analysis times compared to the initial prototype system. The calibration of the new system is demonstrated in scattering studies to determine the size distribution of polystyrene microspheres in a turbid sample. The process for determining the size of cell nuclei is discussed by analyzing measurements of basal cells in a sub-surface layer of intact, unstained epithelial tissue.

Full Text

Duke Authors

Cited Authors

  • Pyhtila, J; Graf, R; Wax, A

Published Date

  • December 2003

Published In

Volume / Issue

  • 11 / 25

Start / End Page

  • 3473 - 3484

PubMed ID

  • 19471481

Pubmed Central ID

  • 19471481

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

International Standard Serial Number (ISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/oe.11.003473

Language

  • eng