Quantification of gas denucleation and thrombogenicity of vascular grafts.

Journal Article (Journal Article)

In vitro methods were developed to measure the air content of vascular graft walls and the thrombogenicity of this air. Gas content (volume %) of expanded polytetrafluoroethylene (ePTFE) grafts from different sources ranged from 75.5 +/- 0.4% to 61.8 +/- 0.3%. Exposure of Vitagraft ePTFE to a vacuum prior to saline immersion replaced 87.5% of the gas nuclei with saline (denucleation). Acetone and ethanol immersion produced 98.9% and 94.3% denucleation, respectively. Denucleation was essentially complete when vacuum exposure was followed by hydrostatic pressure treatment at 500 psig or greater. The influence of gas content on thrombogenicity was determined by immersing graft samples in whole canine blood and weighing the adherent thrombus. Denucleation significantly reduced adherent thrombus weight compared with control grafts (p less than 0.001). Air in Vitagraft walls was responsible for 84% of the adherent thrombus weight at four minutes. The described methods could be employed to assess the hemocompatibility of various biomaterials.

Full Text

Duke Authors

Cited Authors

  • Bensen, CV; Vann, RD; Koger, KE; Klitzman, B

Published Date

  • March 1, 1991

Published In

Volume / Issue

  • 25 / 3

Start / End Page

  • 373 - 386

PubMed ID

  • 2026642

International Standard Serial Number (ISSN)

  • 0021-9304

Digital Object Identifier (DOI)

  • 10.1002/jbm.820250309


  • eng

Conference Location

  • United States