Ig VH hypermutation is absent in the germinal centers of aged mice.

Journal Article

After injection with immunogenic conjugates of the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP), two distinct B cell populations can be identified in the spleen during the primary response. One of these populations is specialized for Ab production; the other, the germinal centers (GCs), has been identified as the site of Ig somatic hypermutation. Ag-driven selection of GC B cells bearing mutated receptors with higher affinity leads to the affinity maturation of serum Ab and increased protective humoral immunity. Microdissection of GC B cell populations specific for NP and sequencing of the recovered Ig heavy chain variable region genes revealed that the somatic hypermutation process is absent in the GCs of aged C57BL/6 mice. However, selection for Ag appears to occur in the absence of hypermutation in the form of competition between unmutated clones of Ag-activated B lymphocytes. Thus, affinity maturation in these animals is limited to the affinities of Ab encoded by the germline.

Full Text

Duke Authors

Cited Authors

  • Miller, C; Kelsoe, G

Published Date

  • October 1, 1995

Published In

Volume / Issue

  • 155 / 7

Start / End Page

  • 3377 - 3384

PubMed ID

  • 7561032

International Standard Serial Number (ISSN)

  • 0022-1767

Language

  • eng

Conference Location

  • United States