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In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. I. The architecture and dynamics of responding cell populations.

Publication ,  Journal Article
Jacob, J; Kassir, R; Kelsoe, G
Published in: J Exp Med
May 1, 1991

After primary immunization with an immunogenic conjugate of (4-hydroxy-3-nitrophenyl)acetyl, two anatomically and phenotypically distinct populations of antibody-forming cells arise in the spleen. As early as 2 d after immunization, foci of antigen-binding B cells are observed along the periphery of the periarteriolar lymphoid sheaths. These foci expand, occupying as much as 1% of the splenic volume by day 8 of the response. Later, foci grow smaller and are virtually absent from the spleen by day 14. A second responding population, germinal center B cells, appear on day 8-10 and persist at least until day 16 post-immunization. Individual foci and germinal centers represent discrete pauciclonal populations that apparently undergo somatic evolution in the course of the primary response. We suggest that foci may represent regions of predominantly interclonal competition for antigen among unmutated B cells, while germinal centers are sites of intraclonal clonal competition between mutated sister lymphocytes.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

May 1, 1991

Volume

173

Issue

5

Start / End Page

1165 / 1175

Location

United States

Related Subject Headings

  • Spleen
  • Phenylacetates
  • Phenotype
  • Nucleic Acid Hybridization
  • Nitrophenols
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Immunology
 

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Jacob, J., Kassir, R., & Kelsoe, G. (1991). In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. I. The architecture and dynamics of responding cell populations. J Exp Med, 173(5), 1165–1175. https://doi.org/10.1084/jem.173.5.1165
Jacob, J., R. Kassir, and G. Kelsoe. “In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. I. The architecture and dynamics of responding cell populations.J Exp Med 173, no. 5 (May 1, 1991): 1165–75. https://doi.org/10.1084/jem.173.5.1165.
Jacob, J., et al. “In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. I. The architecture and dynamics of responding cell populations.J Exp Med, vol. 173, no. 5, May 1991, pp. 1165–75. Pubmed, doi:10.1084/jem.173.5.1165.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

May 1, 1991

Volume

173

Issue

5

Start / End Page

1165 / 1175

Location

United States

Related Subject Headings

  • Spleen
  • Phenylacetates
  • Phenotype
  • Nucleic Acid Hybridization
  • Nitrophenols
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Immunology