An integrated YAC map of the human X chromosome.

Journal Article (Journal Article)

The human X chromosome is associated with a large number of disease phenotypes, principally because of its unique mode of inheritance that tends to reveal all recessive disorders in males. With the longer term goal of identifying and characterizing most of these genes, we have adopted a chromosome-wide strategy to establish a YAC contig map. We have performed > 3250 inter Alu-PCR product hybridizations to identify overlaps between YAC clones. Positional information associated with many of these YAC clones has been derived from our Reference Library Database and a variety of other public sources. We have constructed a YAC contig map of the X chromosome covering 125 Mb of DNA in 25 contigs and containing 906 YAC clones. These contigs have been verified extensively by FISH and by gel and hybridization fingerprinting techniques. This independently derived map exceeds the coverage of recently reported X chromosome maps built as part of whole-genome YAC maps.

Full Text

Duke Authors

Cited Authors

  • Roest Crollius, H; Ross, MT; Grigoriev, A; Knights, CJ; Holloway, E; Misfud, J; Li, K; Playford, M; Gregory, SG; Humphray, SJ; Coffey, AJ; See, CG; Marsh, S; Vatcheva, R; Kumlien, J; Labella, T; Lam, V; Rak, KH; Todd, K; Mott, R; Graeser, D; Rappold, G; Zehetner, G; Poustka, A; Bentley, DR; Monaco, AP; Lehrach, H

Published Date

  • October 1996

Published In

Volume / Issue

  • 6 / 10

Start / End Page

  • 943 - 955

PubMed ID

  • 8908513

International Standard Serial Number (ISSN)

  • 1088-9051

Digital Object Identifier (DOI)

  • 10.1101/gr.6.10.943


  • eng

Conference Location

  • United States