The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X.

Journal Article (Journal Article)

We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.

Full Text

Duke Authors

Cited Authors

  • Bentley, DR; Deloukas, P; Dunham, A; French, L; Gregory, SG; Humphray, SJ; Mungall, AJ; Ross, MT; Carter, NP; Dunham, I; Scott, CE; Ashcroft, KJ; Atkinson, AL; Aubin, K; Beare, DM; Bethel, G; Brady, N; Brook, JC; Burford, DC; Burrill, WD; Burrows, C; Butler, AP; Carder, C; Catanese, JJ; Clee, CM; Clegg, SM; Cobley, V; Coffey, AJ; Cole, CG; Collins, JE; Conquer, JS; Cooper, RA; Culley, KM; Dawson, E; Dearden, FL; Durbin, RM; de Jong, PJ; Dhami, PD; Earthrowl, ME; Edwards, CA; Evans, RS; Gillson, CJ; Ghori, J; Green, L; Gwilliam, R; Halls, KS; Hammond, S; Harper, GL; Heathcott, RW; Holden, JL; Holloway, E; Hopkins, BL; Howard, PJ; Howell, GR; Huckle, EJ; Hughes, J; Hunt, PJ; Hunt, SE; Izmajlowicz, M; Jones, CA; Joseph, SS; Laird, G; Langford, CF; Lehvaslaiho, MH; Leversha, MA; McCann, OT; McDonald, LM; McDowall, J; Maslen, GL; Mistry, D; Moschonas, NK; Neocleous, V; Pearson, DM; Phillips, KJ; Porter, KM; Prathalingam, SR; Ramsey, YH; Ranby, SA; Rice, CM; Rogers, J; Rogers, LJ; Sarafidou, T; Scott, DJ; Sharp, GJ; Shaw-Smith, CJ; Smink, LJ; Soderlund, C; Sotheran, EC; Steingruber, HE; Sulston, JE; Taylor, A; Taylor, RG; Thorpe, AA; Tinsley, E; Warry, GL; Whittaker, A; Whittaker, P; Williams, SH; Wilmer, TE; Wooster, R; Wright, CL

Published Date

  • February 15, 2001

Published In

Volume / Issue

  • 409 / 6822

Start / End Page

  • 942 - 943

PubMed ID

  • 11237015

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/35057165


  • eng

Conference Location

  • England