The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X.
We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.
Bentley, DR; Deloukas, P; Dunham, A; French, L; Gregory, SG; Humphray, SJ; Mungall, AJ; Ross, MT; Carter, NP; Dunham, I; Scott, CE; Ashcroft, KJ; Atkinson, AL; Aubin, K; Beare, DM; Bethel, G; Brady, N; Brook, JC; Burford, DC; Burrill, WD; Burrows, C; Butler, AP; Carder, C; Catanese, JJ; Clee, CM; Clegg, SM; Cobley, V; Coffey, AJ; Cole, CG; Collins, JE; Conquer, JS; Cooper, RA; Culley, KM; Dawson, E; Dearden, FL; Durbin, RM; de Jong, PJ; Dhami, PD; Earthrowl, ME; Edwards, CA; Evans, RS; Gillson, CJ; Ghori, J; Green, L; Gwilliam, R; Halls, KS; Hammond, S; Harper, GL; Heathcott, RW; Holden, JL; Holloway, E; Hopkins, BL; Howard, PJ; Howell, GR; Huckle, EJ; Hughes, J; Hunt, PJ; Hunt, SE; Izmajlowicz, M; Jones, CA; Joseph, SS; Laird, G; Langford, CF; Lehvaslaiho, MH; Leversha, MA; McCann, OT; McDonald, LM; McDowall, J; Maslen, GL; Mistry, D; Moschonas, NK; Neocleous, V; Pearson, DM; Phillips, KJ; Porter, KM; Prathalingam, SR; Ramsey, YH; Ranby, SA; Rice, CM; Rogers, J; Rogers, LJ; Sarafidou, T; Scott, DJ; Sharp, GJ; Shaw-Smith, CJ; Smink, LJ; Soderlund, C; Sotheran, EC; Steingruber, HE; Sulston, JE; Taylor, A; Taylor, RG; Thorpe, AA; Tinsley, E; Warry, GL; Whittaker, A; Whittaker, P; Williams, SH; Wilmer, TE; Wooster, R; Wright, CL
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