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A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region.

Publication ,  Journal Article
Carpten, JD; Makalowska, I; Robbins, CM; Scott, N; Sood, R; Connors, TD; Bonner, TI; Smith, JR; Faruque, MU; Stephan, DA; Pinkett, H; Su, K ...
Published in: Genomics
February 15, 2000

Several hereditary disease loci have been genetically mapped to the chromosome 1q24-q31 interval, including the hereditary prostate cancer 1 (HPC1) locus. Here, we report the construction of a 20-Mb yeast artificial chromosome contig and a high-resolution 6-Mb sequence-ready bacterial artificial chromosome (BAC)/P1-derived artificial chromosome (PAC) contig of 1q25 by sequence and computational analysis, STS content mapping, and chromosome walking. One hundred thirty-six new STSs, including 10 novel simple sequence repeat polymorphisms that are being used for genetic refinement of multiple disease loci, have been generated from this contig and are shown to map to the 1q25 interval. The integrity of the 6-Mb BAC/PAC contig has been confirmed by restriction fingerprinting, and this contig is being used as a template for human chromosome 1 genome sequencing. A transcription mapping effort has resulted in the precise localization of 18 known genes and 31 ESTs by database searching, exon trapping, direct cDNA hybridization, and sample sequencing of BACs from the 1q25 contig. An additional 11 known genes and ESTs have been placed within the larger 1q24-q31 interval. These transcription units represent candidate genes for multiple hereditary diseases, including HPC1.

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Published In

Genomics

DOI

ISSN

0888-7543

Publication Date

February 15, 2000

Volume

64

Issue

1

Start / End Page

1 / 14

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Prostatic Neoplasms
  • Polymorphism, Genetic
  • Physical Chromosome Mapping
  • Molecular Sequence Data
  • Male
  • Humans
  • Genetics & Heredity
  • Genetic Predisposition to Disease
 

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Carpten, J. D., Makalowska, I., Robbins, C. M., Scott, N., Sood, R., Connors, T. D., … Trent, J. M. (2000). A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region. Genomics, 64(1), 1–14. https://doi.org/10.1006/geno.1999.6051
Carpten, J. D., I. Makalowska, C. M. Robbins, N. Scott, R. Sood, T. D. Connors, T. I. Bonner, et al. “A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region.Genomics 64, no. 1 (February 15, 2000): 1–14. https://doi.org/10.1006/geno.1999.6051.
Carpten JD, Makalowska I, Robbins CM, Scott N, Sood R, Connors TD, et al. A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region. Genomics. 2000 Feb 15;64(1):1–14.
Carpten, J. D., et al. “A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region.Genomics, vol. 64, no. 1, Feb. 2000, pp. 1–14. Pubmed, doi:10.1006/geno.1999.6051.
Carpten JD, Makalowska I, Robbins CM, Scott N, Sood R, Connors TD, Bonner TI, Smith JR, Faruque MU, Stephan DA, Pinkett H, Morgenbesser SD, Su K, Graham C, Gregory SG, Williams H, McDonald L, Baxevanis AD, Klingler KW, Landes GM, Trent JM. A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region. Genomics. 2000 Feb 15;64(1):1–14.
Journal cover image

Published In

Genomics

DOI

ISSN

0888-7543

Publication Date

February 15, 2000

Volume

64

Issue

1

Start / End Page

1 / 14

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Prostatic Neoplasms
  • Polymorphism, Genetic
  • Physical Chromosome Mapping
  • Molecular Sequence Data
  • Male
  • Humans
  • Genetics & Heredity
  • Genetic Predisposition to Disease