Detailed molecular analysis of 1p36 in neuroblastoma.

Published

Journal Article

BACKGROUND: Several lines of evidence es tablish that chromosome band 1p36 is frequently deleted in neuroblastoma primary tumors and cell lines, suggesting that a tumor suppressor gene within this region is involved in the development of this tumor. PROCEDURE: We analyzed the status of 1p36 in primary neuroblastomas and cell lines to define the region of consistent rearrangement. RESULTS: Loss of heterozygosity (LOH) studies of primary neuro blastomas identified allelic loss in 135 of 503 tumors (27%), with the smallest region of overlap (SRO) defined distal to D15214 (1p36.3). No homozygous deletions were detected at 120 loci mapping to 1p36.1-p36.3 in a panel of 46 neuroblastoma cell lines. A recently identified patient with neuroblastoma was found to have a constitutional deletion within 1p36.2-p36.3, and this deletion, when combined with the LOH results, defined a smaller SRO of one megabase within 1p36.3. We constructed a comprehensive integrated map of chromosome 1 containing 11,000 markers and large-insert clones, a high-resolution radiation hybrid (RH) map of 1p36, and a P1-artificial chromosome (PAC) contig spanning the SRO, to further characterize the region of interest. Over 768 kb (75%) of the SRO has been sequenced to completion. Further analysis of distal 1p identified 113 transcripts localizing to 1p36, 21 of which were mapped within the SRO. CONCLUSION: This analysis will identify suitable positional candidate transcripts for mutational screening and subsequent identification of the 1p36.3 neuroblastoma suppressor gene.

Full Text

Duke Authors

Cited Authors

  • White, PS; Thompson, PM; Seifried, BA; Sulman, EP; Jensen, SJ; Guo, C; Maris, JM; Hogarty, MD; Allen, C; Biegel, JA; Matise, TC; Gregory, SG; Reynolds, CP; Brodeur, GM

Published Date

  • January 2001

Published In

Volume / Issue

  • 36 / 1

Start / End Page

  • 37 - 41

PubMed ID

  • 11464901

Pubmed Central ID

  • 11464901

International Standard Serial Number (ISSN)

  • 0098-1532

Digital Object Identifier (DOI)

  • 10.1002/1096-911X(20010101)36:1<37::AID-MPO1010>3.0.CO;2-L

Language

  • eng

Conference Location

  • United States