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Detailed molecular analysis of 1p36 in neuroblastoma.

Publication ,  Journal Article
White, PS; Thompson, PM; Seifried, BA; Sulman, EP; Jensen, SJ; Guo, C; Maris, JM; Hogarty, MD; Allen, C; Biegel, JA; Matise, TC; Gregory, SG ...
Published in: Med Pediatr Oncol
January 2001

BACKGROUND: Several lines of evidence es tablish that chromosome band 1p36 is frequently deleted in neuroblastoma primary tumors and cell lines, suggesting that a tumor suppressor gene within this region is involved in the development of this tumor. PROCEDURE: We analyzed the status of 1p36 in primary neuroblastomas and cell lines to define the region of consistent rearrangement. RESULTS: Loss of heterozygosity (LOH) studies of primary neuro blastomas identified allelic loss in 135 of 503 tumors (27%), with the smallest region of overlap (SRO) defined distal to D15214 (1p36.3). No homozygous deletions were detected at 120 loci mapping to 1p36.1-p36.3 in a panel of 46 neuroblastoma cell lines. A recently identified patient with neuroblastoma was found to have a constitutional deletion within 1p36.2-p36.3, and this deletion, when combined with the LOH results, defined a smaller SRO of one megabase within 1p36.3. We constructed a comprehensive integrated map of chromosome 1 containing 11,000 markers and large-insert clones, a high-resolution radiation hybrid (RH) map of 1p36, and a P1-artificial chromosome (PAC) contig spanning the SRO, to further characterize the region of interest. Over 768 kb (75%) of the SRO has been sequenced to completion. Further analysis of distal 1p identified 113 transcripts localizing to 1p36, 21 of which were mapped within the SRO. CONCLUSION: This analysis will identify suitable positional candidate transcripts for mutational screening and subsequent identification of the 1p36.3 neuroblastoma suppressor gene.

Duke Scholars

Published In

Med Pediatr Oncol

DOI

ISSN

0098-1532

Publication Date

January 2001

Volume

36

Issue

1

Start / End Page

37 / 41

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Oncology & Carcinogenesis
  • Neuroblastoma
  • Microsatellite Repeats
  • Loss of Heterozygosity
  • Infant
  • In Situ Hybridization, Fluorescence
  • Humans
  • Genotype
  • Genes, Tumor Suppressor
 

Citation

APA
Chicago
ICMJE
MLA
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White, P. S., Thompson, P. M., Seifried, B. A., Sulman, E. P., Jensen, S. J., Guo, C., … Brodeur, G. M. (2001). Detailed molecular analysis of 1p36 in neuroblastoma. Med Pediatr Oncol, 36(1), 37–41. https://doi.org/10.1002/1096-911X(20010101)36:1<37::AID-MPO1010>3.0.CO;2-L
White, P. S., P. M. Thompson, B. A. Seifried, E. P. Sulman, S. J. Jensen, C. Guo, J. M. Maris, et al. “Detailed molecular analysis of 1p36 in neuroblastoma.Med Pediatr Oncol 36, no. 1 (January 2001): 37–41. https://doi.org/10.1002/1096-911X(20010101)36:1<37::AID-MPO1010>3.0.CO;2-L.
White PS, Thompson PM, Seifried BA, Sulman EP, Jensen SJ, Guo C, et al. Detailed molecular analysis of 1p36 in neuroblastoma. Med Pediatr Oncol. 2001 Jan;36(1):37–41.
White, P. S., et al. “Detailed molecular analysis of 1p36 in neuroblastoma.Med Pediatr Oncol, vol. 36, no. 1, Jan. 2001, pp. 37–41. Pubmed, doi:10.1002/1096-911X(20010101)36:1<37::AID-MPO1010>3.0.CO;2-L.
White PS, Thompson PM, Seifried BA, Sulman EP, Jensen SJ, Guo C, Maris JM, Hogarty MD, Allen C, Biegel JA, Matise TC, Gregory SG, Reynolds CP, Brodeur GM. Detailed molecular analysis of 1p36 in neuroblastoma. Med Pediatr Oncol. 2001 Jan;36(1):37–41.

Published In

Med Pediatr Oncol

DOI

ISSN

0098-1532

Publication Date

January 2001

Volume

36

Issue

1

Start / End Page

37 / 41

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Oncology & Carcinogenesis
  • Neuroblastoma
  • Microsatellite Repeats
  • Loss of Heterozygosity
  • Infant
  • In Situ Hybridization, Fluorescence
  • Humans
  • Genotype
  • Genes, Tumor Suppressor