An integrated physical map of 210 markers assigned to the short arm of human chromosome 11.

Published

Journal Article

Using a panel of patient cell lines with chromosomal breakpoints, we constructed a physical map for the short arm of human chromosome 11. We focused on 11p15, a chromosome band harboring at least 25 known genes and associated with the Beckwith-Wiedemann syndrome, several childhood tumors, and genomic imprinting. This underlines the need for a physical map for this region. We divided the short arm of chromosome 11 into 18 breakpoint regions, and a large series of new and previously described genes and markers was mapped within these intervals using fluorescence in situ hybridization. Cosmid fingerprint analysis showed that 19 of these markers were included in cosmid contigs. A detailed 10-Mb pulsed-field physical map of the region 11p15.3-pter was constructed. These three different approaches enabled the high-resolution mapping of 210 markers, including 22 known genes.

Full Text

Duke Authors

Cited Authors

  • Redeker, E; Hoovers, JM; Alders, M; van Moorsel, CJ; Ivens, AC; Gregory, S; Kalikin, L; Bliek, J; de Galan, L; van den Bogaard, R

Published Date

  • June 1, 1994

Published In

Volume / Issue

  • 21 / 3

Start / End Page

  • 538 - 550

PubMed ID

  • 7959730

Pubmed Central ID

  • 7959730

International Standard Serial Number (ISSN)

  • 0888-7543

Language

  • eng

Conference Location

  • United States