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Definition and characterization of a region of 1p36.3 consistently deleted in neuroblastoma.

Publication ,  Journal Article
White, PS; Thompson, PM; Gotoh, T; Okawa, ER; Igarashi, J; Kok, M; Winter, C; Gregory, SG; Hogarty, MD; Maris, JM; Brodeur, GM
Published in: Oncogene
April 14, 2005

Substantial genomic and functional evidence from primary tumors and cell lines indicates that a consistent region of distal chromosome 1p is deleted in a sizable proportion of human neuroblastomas, suggesting that this region contains one or more tumor suppressor genes. To determine systematically and precisely the location and extent of 1p deletion in neuroblastomas, we performed allelic loss studies of 737 primary neuroblastomas and genotype analysis of 46 neuroblastoma cell lines. Together, the results defined a single region within 1p36.3 that was consistently deleted in 25% of tumors and 87% of cell lines. Two neuroblastoma patients had constitutional deletions of distal 1p36 that overlapped the tumor-defined region. The tumor- and constitutionally-derived deletions together defined a smallest region of consistent deletion (SRD) between D1S2795 and D1S253. The 1p36.3 SRD was deleted in all but one of the 184 tumors with 1p deletion. Physical mapping and DNA sequencing determined that the SRD minimally spans an estimated 729 kb. Genomic content and sequence analysis of the SRD identified 15 characterized, nine uncharacterized, and six predicted genes in the region. The RNA expression profiles of 21 of the genes were investigated in a variety of normal tissues. The SHREW1 and KCNAB2 genes both had tissue-restricted expression patterns, including expression in the nervous system. In addition, a novel gene (CHD5) with strong homology to proteins involved in chromatin remodeling was expressed mainly in neural tissues. Together, these results suggest that one or more genes involved in neuroblastoma tumorigenesis or tumor progression are likely contained within this region.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

April 14, 2005

Volume

24

Issue

16

Start / End Page

2684 / 2694

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Physical Chromosome Mapping
  • Oncology & Carcinogenesis
  • Neuroblastoma
  • Loss of Heterozygosity
  • Humans
  • Genotype
  • Genetic Markers
  • Genes, Tumor Suppressor
  • Gene Expression Profiling
 

Citation

APA
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ICMJE
MLA
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White, P. S., Thompson, P. M., Gotoh, T., Okawa, E. R., Igarashi, J., Kok, M., … Brodeur, G. M. (2005). Definition and characterization of a region of 1p36.3 consistently deleted in neuroblastoma. Oncogene, 24(16), 2684–2694. https://doi.org/10.1038/sj.onc.1208306
White, Peter S., Patricia M. Thompson, Takahiro Gotoh, Erin R. Okawa, Jun Igarashi, Marleen Kok, Cynthia Winter, et al. “Definition and characterization of a region of 1p36.3 consistently deleted in neuroblastoma.Oncogene 24, no. 16 (April 14, 2005): 2684–94. https://doi.org/10.1038/sj.onc.1208306.
White PS, Thompson PM, Gotoh T, Okawa ER, Igarashi J, Kok M, et al. Definition and characterization of a region of 1p36.3 consistently deleted in neuroblastoma. Oncogene. 2005 Apr 14;24(16):2684–94.
White, Peter S., et al. “Definition and characterization of a region of 1p36.3 consistently deleted in neuroblastoma.Oncogene, vol. 24, no. 16, Apr. 2005, pp. 2684–94. Pubmed, doi:10.1038/sj.onc.1208306.
White PS, Thompson PM, Gotoh T, Okawa ER, Igarashi J, Kok M, Winter C, Gregory SG, Hogarty MD, Maris JM, Brodeur GM. Definition and characterization of a region of 1p36.3 consistently deleted in neuroblastoma. Oncogene. 2005 Apr 14;24(16):2684–2694.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

April 14, 2005

Volume

24

Issue

16

Start / End Page

2684 / 2694

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Physical Chromosome Mapping
  • Oncology & Carcinogenesis
  • Neuroblastoma
  • Loss of Heterozygosity
  • Humans
  • Genotype
  • Genetic Markers
  • Genes, Tumor Suppressor
  • Gene Expression Profiling