Protein-protein interactions contributing to the specificity of intracellular vesicular trafficking.


Journal Article

Intracellular vesicles destined to fuse with the plasma membrane and secrete their contents must have a mechanism for specifically interacting with the appropriate target membrane. Such a mechanism is now suggested by the demonstration of specific interaction between vesicular proteins and plasma membrane proteins. The vesicle-associated membrane proteins (VAMPs) 1 and 2 specifically bind the acceptor membrane proteins syntaxin 1A and 4 but not syntaxin 2 or 3. The binding site is within amino acids 194 to 267 of syntaxin 1A, and the approximate equilibrium dissociation constants is 4.7 x 10(-6) molar. These data suggest a physical basis for the specificity of intracellular vesicular transport.

Full Text

Duke Authors

Cited Authors

  • Calakos, N; Bennett, MK; Peterson, KE; Scheller, RH

Published Date

  • February 25, 1994

Published In

Volume / Issue

  • 263 / 5150

Start / End Page

  • 1146 - 1149

PubMed ID

  • 8108733

Pubmed Central ID

  • 8108733

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.8108733


  • eng

Conference Location

  • United States