Promoter-specific trans-activation by the adenovirus E1A12S product involves separate E1A domains.

Published

Journal Article

Recent studies have shown that the adenovirus E1A12S product can trans-activate transcription by activating the transcription factor E2F. However, E2F cannot be the only target for the E1A12S product, since several cellular promoters have been found to be activated by the E1A12S protein even though they lack E2F sites. Indeed, we now show that activation of the hsp70 promoter by the E1A12S product requires the TATAA sequence. Moreover, activation of the hsp70 promoter requires the N-terminal domain of the E1A protein and does not require the conserved region 2 sequences which are required for the E2F-dependent activation of transcription. We conclude that the targeting of distinct transcription factors, leading to trans-activation of transcription of multiple promoters, involves distinct domains of the E1A proteins that are also required for oncogenic activity.

Full Text

Duke Authors

Cited Authors

  • Kraus, VB; Moran, E; Nevins, JR

Published Date

  • October 1992

Published In

Volume / Issue

  • 12 / 10

Start / End Page

  • 4391 - 4399

PubMed ID

  • 1406628

Pubmed Central ID

  • 1406628

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.12.10.4391

Language

  • eng