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Osmotic loading to determine the intrinsic material properties of guinea pig knee cartilage.

Publication ,  Journal Article
Flahiff, CM; Narmoneva, DA; Huebner, JL; Kraus, VB; Guilak, F; Setton, LA
Published in: J Biomech
September 2002

Few methods exist to study cartilage mechanics in small animal joints due to the difficulties associated with handling small tissue samples. In this study, we apply an osmotic loading method to quantify the intrinsic material properties of articular cartilage in small animal joints. Cartilage samples were studied from the femoral condyle and tibial plateau of two-month old guinea pigs. Swelling strains were measured using confocal fluorescence scanning microscopy in samples subjected to osmotic loading. A histochemical staining method was developed and calibrated for quantification of negative fixed charge density in guinea pig cartilage. Site-matched swelling strain data and fixed charge density values were then used with a triphasic theoretical model for cartilage swelling to determine the uniaxial modulus of the cartilage solid matrix. Moduli obtained in this study (7.2 MPa femoral condyle; 10.8 MPa, tibial plateau) compare well with previously reported values for the tensile moduli of human and other animal cartilages determined from uniaxial tension experiments. This study provides the first available data for material properties and fixed charge density in cartilage from the guinea pig knee and suggests a promising method for tracking changes in cartilage mechanics in small animal models of degeneration.

Duke Scholars

Published In

J Biomech

DOI

ISSN

0021-9290

Publication Date

September 2002

Volume

35

Issue

9

Start / End Page

1285 / 1290

Location

United States

Related Subject Headings

  • Tibia
  • Stress, Mechanical
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Patella
  • Osmotic Pressure
  • Microscopy, Fluorescence
  • Microscopy, Confocal
  • Male
  • Knee Joint
 

Citation

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ICMJE
MLA
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Flahiff, C. M., Narmoneva, D. A., Huebner, J. L., Kraus, V. B., Guilak, F., & Setton, L. A. (2002). Osmotic loading to determine the intrinsic material properties of guinea pig knee cartilage. J Biomech, 35(9), 1285–1290. https://doi.org/10.1016/s0021-9290(02)00079-9
Flahiff, Charlene M., Daria A. Narmoneva, Janet L. Huebner, Virginia B. Kraus, Farshid Guilak, and Lori A. Setton. “Osmotic loading to determine the intrinsic material properties of guinea pig knee cartilage.J Biomech 35, no. 9 (September 2002): 1285–90. https://doi.org/10.1016/s0021-9290(02)00079-9.
Flahiff CM, Narmoneva DA, Huebner JL, Kraus VB, Guilak F, Setton LA. Osmotic loading to determine the intrinsic material properties of guinea pig knee cartilage. J Biomech. 2002 Sep;35(9):1285–90.
Flahiff, Charlene M., et al. “Osmotic loading to determine the intrinsic material properties of guinea pig knee cartilage.J Biomech, vol. 35, no. 9, Sept. 2002, pp. 1285–90. Pubmed, doi:10.1016/s0021-9290(02)00079-9.
Flahiff CM, Narmoneva DA, Huebner JL, Kraus VB, Guilak F, Setton LA. Osmotic loading to determine the intrinsic material properties of guinea pig knee cartilage. J Biomech. 2002 Sep;35(9):1285–1290.
Journal cover image

Published In

J Biomech

DOI

ISSN

0021-9290

Publication Date

September 2002

Volume

35

Issue

9

Start / End Page

1285 / 1290

Location

United States

Related Subject Headings

  • Tibia
  • Stress, Mechanical
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Patella
  • Osmotic Pressure
  • Microscopy, Fluorescence
  • Microscopy, Confocal
  • Male
  • Knee Joint