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Modulation of cell growth and differentiation by ceramide.

Publication ,  Journal Article
Bielawska, A; Linardic, CM; Hannun, YA
Published in: FEBS Lett
July 28, 1992

Ceramide has been suggested as an intracellular modulator of cell growth and differentiation [Okazaki, T. et al. (1990) J. Biol. Chem. 265, 15823-15831]. In this study, parameters that modulate the effects of ceramide on HL-60 cell growth and differentiation were examined. A short-chain, cell-permeable analog of ceramide, C2-ceramide, induced differentiation of HL-60 human leukemia cells and inhibited HL-60 growth in a concentration-dependent manner. The potency of C2-ceramide was modulated by the starting cell density such that the concentration of C2-ceramide producing 50% inhibition of cell growth (IC50%) ranged from 2 microM (for cells suspended at 1 x 10(5) cells/ml) to 11 microM (for cells at 8 x 10(5) cells/ml). However, the IC50% showed little variation if the concentration of C2-ceramide was expressed as fmol of C2-ceramide per 10(5) cells. Therefore, the effectiveness of C2-ceramide appeared to be primarily determined by its cellular rather than molar concentration. Binding of C2-ceramide to serum proteins resulted in a 10-fold increase in the IC50%. These results demonstrate that the biologic activity of C2-ceramide is subject to surface dilution kinetics and is sensitive to the presence of lipid-binding proteins. In these properties, ceramide behaves as a prototypic lipid second messenger/intracellular mediator.

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Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

July 28, 1992

Volume

307

Issue

2

Start / End Page

211 / 214

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Leukemia, Myeloid
  • Humans
  • Ceramides
  • Cell Division
  • Cell Differentiation
  • Cell Count
  • Biochemistry & Molecular Biology
  • 3101 Biochemistry and cell biology
  • 0603 Evolutionary Biology
 

Citation

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Bielawska, A., Linardic, C. M., & Hannun, Y. A. (1992). Modulation of cell growth and differentiation by ceramide. FEBS Lett, 307(2), 211–214. https://doi.org/10.1016/0014-5793(92)80769-d
Bielawska, A., C. M. Linardic, and Y. A. Hannun. “Modulation of cell growth and differentiation by ceramide.FEBS Lett 307, no. 2 (July 28, 1992): 211–14. https://doi.org/10.1016/0014-5793(92)80769-d.
Bielawska A, Linardic CM, Hannun YA. Modulation of cell growth and differentiation by ceramide. FEBS Lett. 1992 Jul 28;307(2):211–4.
Bielawska, A., et al. “Modulation of cell growth and differentiation by ceramide.FEBS Lett, vol. 307, no. 2, July 1992, pp. 211–14. Pubmed, doi:10.1016/0014-5793(92)80769-d.
Bielawska A, Linardic CM, Hannun YA. Modulation of cell growth and differentiation by ceramide. FEBS Lett. 1992 Jul 28;307(2):211–214.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

July 28, 1992

Volume

307

Issue

2

Start / End Page

211 / 214

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Leukemia, Myeloid
  • Humans
  • Ceramides
  • Cell Division
  • Cell Differentiation
  • Cell Count
  • Biochemistry & Molecular Biology
  • 3101 Biochemistry and cell biology
  • 0603 Evolutionary Biology