2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region.

Journal Article

We have determined the 2.0 A crystal structure of a fragment of human fibronectin encompassing the seventh through the RGD-containing tenth type III repeats (FN7-10). The structure reveals an extended rod-like molecule with a long axis of approximately 140 A and highly variable relationships between adjacent domains. An unusually small rotation between domains 9 and 10 creates a distinctive binding site, in which the RGD loop from domain 10 and the "synergy" region from domain 9 are on the same face of FN7-10 and thus easily accessible to a single integrin molecule. The cell-binding RGD loop is well-ordered in this structure and extends approximately 10 A away from the FN7-10 core.

Full Text

Duke Authors

Cited Authors

  • Leahy, DJ; Aukhil, I; Erickson, HP

Published Date

  • January 12, 1996

Published In

Volume / Issue

  • 84 / 1

Start / End Page

  • 155 - 164

PubMed ID

  • 8548820

International Standard Serial Number (ISSN)

  • 0092-8674

Language

  • eng

Conference Location

  • United States