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Functional heterogeneity of mammalian TATA-box sequences revealed by interaction with a cell-specific enhancer.

Publication ,  Journal Article
Wefald, FC; Devlin, BH; Williams, RS
Published in: Nature
March 15, 1990

A regulatory element upstream of the human myoglobin gene functions as a muscle-specific enhancer (MSE) in conjunction with core promoter elements of the myoglobin gene, but not in combination with the simian virus 40 (SV40) early promoter. These two promoters differ in the sequences of their 'TATA boxes': for the myoglobin gene, the sequence is TATAAAA, whereas for SV40, the sequence is TATTTAT. We have now tested the hypothesis that this sequence difference is responsible for the differential response of the promoters to the MSE. We found that when the TATA box sequence of the myoglobin promoter was changed to that of the SV40 promoter, responsiveness to the MSE was abolished; conversely, when the SV40 TATA box sequence was changed to that of the myoglobin promoter, the promoter became responsive to the MSE. We conclude that mammalian TATA-box elements are functionally heterogeneous, and suggest that this heterogeneity reflects differential interactions with distinctive TATA box-binding factors, only some of which can act cooperatively with MSE-binding proteins to generate an active transcriptional complex.

Duke Scholars

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

March 15, 1990

Volume

344

Issue

6263

Start / End Page

260 / 262

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Simian virus 40
  • Regulatory Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Myoglobin
  • Mutation
  • Isoenzymes
  • Humans
  • General Science & Technology
  • Gene Expression
 

Citation

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Wefald, F. C., Devlin, B. H., & Williams, R. S. (1990). Functional heterogeneity of mammalian TATA-box sequences revealed by interaction with a cell-specific enhancer. Nature, 344(6263), 260–262. https://doi.org/10.1038/344260a0
Wefald, F. C., B. H. Devlin, and R. S. Williams. “Functional heterogeneity of mammalian TATA-box sequences revealed by interaction with a cell-specific enhancer.Nature 344, no. 6263 (March 15, 1990): 260–62. https://doi.org/10.1038/344260a0.
Wefald FC, Devlin BH, Williams RS. Functional heterogeneity of mammalian TATA-box sequences revealed by interaction with a cell-specific enhancer. Nature. 1990 Mar 15;344(6263):260–2.
Wefald, F. C., et al. “Functional heterogeneity of mammalian TATA-box sequences revealed by interaction with a cell-specific enhancer.Nature, vol. 344, no. 6263, Mar. 1990, pp. 260–62. Pubmed, doi:10.1038/344260a0.
Wefald FC, Devlin BH, Williams RS. Functional heterogeneity of mammalian TATA-box sequences revealed by interaction with a cell-specific enhancer. Nature. 1990 Mar 15;344(6263):260–262.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

March 15, 1990

Volume

344

Issue

6263

Start / End Page

260 / 262

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Simian virus 40
  • Regulatory Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Myoglobin
  • Mutation
  • Isoenzymes
  • Humans
  • General Science & Technology
  • Gene Expression