Molecular and structural analysis of nuclear localizing anti-DNA lupus antibodies.

Published

Journal Article

To determine the structure of three nuclear localizing lupus anti-DNA immunoglobulins (Igs) and to search for clues to mechanisms of cellular and/or nuclear access, their H- and L-chain variable region sequences were determined and subjected to three-dimensional modeling. Although the results indicate heterogeneity in their primary structures, the H chains are encoded by 3 members of the J558 VH gene family with a common tertiary conformation that is not shared by a J558-encoded nonnuclear localizing anti-DNA control Ig. Furthermore, at least two of the Igs share a conformational motif in the H-chain CDR3, and all three Igs contain multiple positively charged amino acids in their CDRs, resembling nuclear localization signals that direct protein nuclear import. Notably, each VH and VK gene is also found recurrently among previously described autoantibodies. Molecular analysis further indicates that both germline-encoded and significantly mutated V genes can generate nuclear localizing anti-DNA Ig.

Full Text

Duke Authors

Cited Authors

  • Foster, MH; Kieber-Emmons, T; Ohliger, M; Madaio, MP

Published Date

  • 1994

Published In

Volume / Issue

  • 13 / 2-3

Start / End Page

  • 186 - 206

PubMed ID

  • 7775809

Pubmed Central ID

  • 7775809

International Standard Serial Number (ISSN)

  • 0257-277X

Language

  • eng

Conference Location

  • United States