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A small yeast RNA blocks hepatitis C virus internal ribosome entry site (HCV IRES)-mediated translation and inhibits replication of a chimeric poliovirus under translational control of the HCV IRES element.

Publication ,  Journal Article
Das, S; Ott, M; Yamane, A; Tsai, W; Gromeier, M; Lahser, F; Gupta, S; Dasgupta, A
Published in: J Virol
July 1998

Hepatitis C virus (HCV) infection frequently leads to chronic hepatitis and cirrhosis of the liver and has been linked to development of hepatocellular carcinoma. We previously identified a small yeast RNA (IRNA) capable of specifically inhibiting poliovirus (PV) internal ribosome entry site (IRES)-mediated translation. Here we report that IRNA specifically inhibits HCV IRES-mediated translation both in vivo and in vitro. A number of human hepatoma (Huh-7) cell lines expressing IRNA were prepared and characterized. Constitutive expression of IRNA was not detrimental to cell growth. HCV IRES-mediated cap-independent translation was markedly inhibited in cells constitutively expressing IRNA compared to control hepatoma cells. However, cap-dependent translation was not significantly affected in these cell lines. Additionally, Huh-7 cells constitutively expressing IRNA became refractory to infection by a PV-HCV chimera in which the PV IRES is replaced by the HCV IRES. In contrast, replication of a PV-encephalomyocarditis virus (EMCV) chimera containing the EMCV IRES element was not affected significantly in the IRNA-producing cell line. Finally, the binding of the La autoantigen to the HCV IRES element was specifically and efficiently competed by IRNA. These results provide a basis for development of novel drugs effective against HCV infection.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

July 1998

Volume

72

Issue

7

Start / End Page

5638 / 5647

Location

United States

Related Subject Headings

  • Yeasts
  • Virus Replication
  • Virology
  • Tumor Cells, Cultured
  • SS-B Antigen
  • Ribonucleoproteins
  • RNA, Fungal
  • Protein Biosynthesis
  • Poliovirus
  • Liver Neoplasms
 

Citation

APA
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ICMJE
MLA
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Das, S., Ott, M., Yamane, A., Tsai, W., Gromeier, M., Lahser, F., … Dasgupta, A. (1998). A small yeast RNA blocks hepatitis C virus internal ribosome entry site (HCV IRES)-mediated translation and inhibits replication of a chimeric poliovirus under translational control of the HCV IRES element. J Virol, 72(7), 5638–5647. https://doi.org/10.1128/JVI.72.7.5638-5647.1998
Das, S., M. Ott, A. Yamane, W. Tsai, M. Gromeier, F. Lahser, S. Gupta, and A. Dasgupta. “A small yeast RNA blocks hepatitis C virus internal ribosome entry site (HCV IRES)-mediated translation and inhibits replication of a chimeric poliovirus under translational control of the HCV IRES element.J Virol 72, no. 7 (July 1998): 5638–47. https://doi.org/10.1128/JVI.72.7.5638-5647.1998.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

July 1998

Volume

72

Issue

7

Start / End Page

5638 / 5647

Location

United States

Related Subject Headings

  • Yeasts
  • Virus Replication
  • Virology
  • Tumor Cells, Cultured
  • SS-B Antigen
  • Ribonucleoproteins
  • RNA, Fungal
  • Protein Biosynthesis
  • Poliovirus
  • Liver Neoplasms