Pseudomonas invasion of type I pneumocytes is dependent on the expression and phosphorylation of caveolin-2.
Journal Article (Journal Article)
Pseudomonas aeruginosa is a major cause of pneumonia in patients with cystic fibrosis and other immuncompromising conditions. Here we showed that P. aeruginosa invades type I pneumocytes via a lipid raft-mediated mechanism. P. aeruginosa invasion of rat primary type I-like pneumocytes as well as a murine lung epithelial cell line 12 (MLE-12) is inhibited by drugs that remove membrane cholesterol and disrupt lipid rafts. Confocal microscopy demonstrated co-localization of intracellular P. aeruginosa with lipid raft components including caveolin-1 and -2. We generated caveolin-1 and -2 knockdowns in MLE-12 cells by using RNA interference techniques. Decreased expression of caveolin-2 significantly impaired the ability of P. aeruginosa to invade MLE-12 cells. In addition, the lipid raft-dependent tyrosine phosphorylation of caveolin-2 appeared to be a critical regulator of P. aeruginosa invasion.
- Zaas, DW; Duncan, MJ; Li, G; Wright, JR; Abraham, SN
- February 11, 2005
Volume / Issue
- 280 / 6
Start / End Page
- 4864 - 4872
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States