Pseudomonas invasion of type I pneumocytes is dependent on the expression and phosphorylation of caveolin-2.

Published

Journal Article

Pseudomonas aeruginosa is a major cause of pneumonia in patients with cystic fibrosis and other immuncompromising conditions. Here we showed that P. aeruginosa invades type I pneumocytes via a lipid raft-mediated mechanism. P. aeruginosa invasion of rat primary type I-like pneumocytes as well as a murine lung epithelial cell line 12 (MLE-12) is inhibited by drugs that remove membrane cholesterol and disrupt lipid rafts. Confocal microscopy demonstrated co-localization of intracellular P. aeruginosa with lipid raft components including caveolin-1 and -2. We generated caveolin-1 and -2 knockdowns in MLE-12 cells by using RNA interference techniques. Decreased expression of caveolin-2 significantly impaired the ability of P. aeruginosa to invade MLE-12 cells. In addition, the lipid raft-dependent tyrosine phosphorylation of caveolin-2 appeared to be a critical regulator of P. aeruginosa invasion.

Full Text

Duke Authors

Cited Authors

  • Zaas, DW; Duncan, MJ; Li, G; Wright, JR; Abraham, SN

Published Date

  • February 11, 2005

Published In

Volume / Issue

  • 280 / 6

Start / End Page

  • 4864 - 4872

PubMed ID

  • 15545264

Pubmed Central ID

  • 15545264

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.M411702200

Language

  • eng

Conference Location

  • United States