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Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes.

Publication ,  Journal Article
Fathman, CG; Pisetsky, DS; Sachs, DH
Published in: J Exp Med
March 1, 1977

Rat antisera raised against anti-nuclease antibodies from mouse strains A/J and SJL detect strain-specific idiotypic determinants related to the antigen-combining site. These antisera have been used to investigate the genetic linkage and strain distribution of the anti-nuclease idiotypes. Despite the existence of an H-2-linked immune response gene controlling the humoral response to nuclease, expression of the A/J anti-nuclease idiotype has been shown to be independent of genes in the H-2 region: the A/J idiotype was present in immune sera from strains A/J (H-2a) and A.BY (H-2b) but absent in sera from strains B10 (H-2b) and B10.A (H-2a). An analysis of the segregation of the A/J idiotype in offspring of the backcross (A/J x B10.A) x B10.A demonstrated linkage to the Ig-1e heavy chain allotype markers. In a small sample of backcross animals a very high apparent recombination frequency was observed, but further backcross analyses and progeny testing of putative recombinant animals will be required to substantiate this observation. Analysis of the A/J and SJL anti-nuclease idiotype markers in the BALB/c, CB.20, and BAB.14 strains indicate that these idiotypic markers may permit mapping of distinct variable region genes.

Duke Scholars

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

March 1, 1977

Volume

145

Issue

3

Start / End Page

569 / 577

Location

United States

Related Subject Headings

  • Species Specificity
  • Rats, Inbred Lew
  • Rats
  • Micrococcal Nuclease
  • Mice, Inbred Strains
  • Mice
  • Immunology
  • Immune Sera
  • Haplotypes
  • Genetic Linkage
 

Citation

APA
Chicago
ICMJE
MLA
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Fathman, C. G., Pisetsky, D. S., & Sachs, D. H. (1977). Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes. J Exp Med, 145(3), 569–577. https://doi.org/10.1084/jem.145.3.569
Fathman, C. G., D. S. Pisetsky, and D. H. Sachs. “Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes.J Exp Med 145, no. 3 (March 1, 1977): 569–77. https://doi.org/10.1084/jem.145.3.569.
Fathman, C. G., et al. “Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes.J Exp Med, vol. 145, no. 3, Mar. 1977, pp. 569–77. Pubmed, doi:10.1084/jem.145.3.569.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

March 1, 1977

Volume

145

Issue

3

Start / End Page

569 / 577

Location

United States

Related Subject Headings

  • Species Specificity
  • Rats, Inbred Lew
  • Rats
  • Micrococcal Nuclease
  • Mice, Inbred Strains
  • Mice
  • Immunology
  • Immune Sera
  • Haplotypes
  • Genetic Linkage