Overview
Studies in my laboratory concern the immunological properties of DNA as related to two main topics: 1) the induction of anti-DNA responses in systemic lupus erythematosus; and 2) the stimulation of innate immunity by bacterial DNA. These topics are closely linked since we have established novel disease models in which lupus-like illness can be induced in normal mice by bacterial DNA under conditions in which mammalian DNA is inactive. This model has been useful in elucidating mechanisms of DNA antigen drive in autoimmunity.
To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine monoclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.
In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.
The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.
To pursue these studies, our laboratory conducts investigations in the following areas: 1) specificity of anti-DNA for epitopes on mammalian and bacterial DNA; 2) molecular analysis of murine monoclonal anti-DNA antibodies; 3) histopathological analyses of DNA-immunized mice; 4) in vitro analysis of proliferation, antibody production and cytokine expression in human and murine immune cells; and 5) analysis of DNA binding to cell surface molecules on B cells, T cells and macrophages. Results of these studies have allowed identification of at least two structural motifs that are immunoactive. We are also exploring the impact of chemical modifications of the DNA backbone.
In addition to work on the immunology of DNA, I am also involved in clinical trials related to new immunomodulatory agents in the treatment of rheumatoid arthritis as well as serological markers of disease activity.
The areas of research for which I am known nationally are anti-DNA antibodies, systemic lupus erythematosus and immunological properties of DNA. I have written textbook chapters and reviews on all these subjects.
Current Appointments & Affiliations
Professor of Medicine
·
1990 - Present
Medicine, Rheumatology and Immunology,
Medicine
Professor of Integrative Immunobiology
·
1994 - Present
Integrative Immunobiology,
Basic Science Departments
Member of the Duke Cancer Institute
·
1978 - Present
Duke Cancer Institute,
Institutes and Centers
Member of the Duke Human Vaccine Institute
·
2014 - Present
Duke Human Vaccine Institute,
Institutes and Centers
Recent Publications
DNA coronas resist nuclease degradation.
Journal Article Biophys J · May 29, 2025 The interaction of cell-free DNA with biological particles has been linked to autoimmune diseases such as systemic lupus erythematosus, but mechanistic details are lacking. Our recent work has shown that DNA adsorbed on the surface of synthetic particles, ... Full text Link to item CiteThe Meaning of Goodbye.
Journal Article N Engl J Med · May 29, 2025 Full text Link to item CiteThe Dynamics of Antinuclear Antibody Production in Systemic Lupus Erythematosus: New Insights for New Therapies.
Journal Article Arthritis Rheumatol · May 12, 2025 Full text Link to item CiteRecent Grants
Complement activating properties of anti-Sm/RNP antibodies in SLE
ResearchCo Investigator · Awarded by Department of Defense · 2024 - 2026Improving Quality of Life in SLE by Stratified Personalized Health Planning
ResearchCo Investigator · Awarded by Department of Defense · 2022 - 2026Biomarkers to distinguish between Type 1 and 2 SLE activity
ResearchCo-Principal Investigator · Awarded by Exagen , Inc. · 2020 - 2026View All Grants
Education, Training & Certifications
Yeshiva University, Albert Einstein College of Medicine ·
1973
M.D.
Yeshiva University, Albert Einstein College of Medicine ·
1972
Ph.D.