Skip to main content

Flow cytometry sorting of recombinant mycobacterial species yields bacterial clones with enhanced insert expression.

Publication ,  Journal Article
Yu, J-S; Whitesides, J; Lee, S-H; Taylor, N; Jacobs, WR; Letvin, NL; Haynes, BF
Published in: Clin Vaccine Immunol
January 2011

Recombinant mycobacteria hold promise as vectors for delivery of HIV-1 and other pathogen antigen inserts for inducing systemic and mucosal immune responses. In general, the immunogenicity of the recombinant mycobacterial insert is proportional to the level of insert expression. In this study, a novel flow cytometry-based assay has been developed to sort live recombinant mycobacterial mutants with high expression of foreign inserts and to enrich those sorted bacterial populations. Sorted recombinant mycobacterial clones expressed higher levels of the ovalbumin SIINFEKL epitope, and select sorted clones showed better immunogenicity than unsorted recombinant mycobacteria. Thus, flow cytometry-based sorting can isolate recombinant mycobacteria enriched for higher insert expression.

Duke Scholars

Published In

Clin Vaccine Immunol

DOI

EISSN

1556-679X

Publication Date

January 2011

Volume

18

Issue

1

Start / End Page

43 / 49

Location

United States

Related Subject Headings

  • Recombinant Fusion Proteins
  • Rabbits
  • Ovalbumin
  • Mycobacterium bovis
  • Mycobacterium
  • Mutation
  • Mutagenesis, Insertional
  • Microbiology
  • Mice, Inbred C57BL
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yu, J.-S., Whitesides, J., Lee, S.-H., Taylor, N., Jacobs, W. R., Letvin, N. L., & Haynes, B. F. (2011). Flow cytometry sorting of recombinant mycobacterial species yields bacterial clones with enhanced insert expression. Clin Vaccine Immunol, 18(1), 43–49. https://doi.org/10.1128/CVI.00292-10
Yu, Jae-Sung, John Whitesides, Sun-Hee Lee, Natalie Taylor, William R. Jacobs, Norman L. Letvin, and Barton F. Haynes. “Flow cytometry sorting of recombinant mycobacterial species yields bacterial clones with enhanced insert expression.Clin Vaccine Immunol 18, no. 1 (January 2011): 43–49. https://doi.org/10.1128/CVI.00292-10.
Yu J-S, Whitesides J, Lee S-H, Taylor N, Jacobs WR, Letvin NL, et al. Flow cytometry sorting of recombinant mycobacterial species yields bacterial clones with enhanced insert expression. Clin Vaccine Immunol. 2011 Jan;18(1):43–9.
Yu, Jae-Sung, et al. “Flow cytometry sorting of recombinant mycobacterial species yields bacterial clones with enhanced insert expression.Clin Vaccine Immunol, vol. 18, no. 1, Jan. 2011, pp. 43–49. Pubmed, doi:10.1128/CVI.00292-10.
Yu J-S, Whitesides J, Lee S-H, Taylor N, Jacobs WR, Letvin NL, Haynes BF. Flow cytometry sorting of recombinant mycobacterial species yields bacterial clones with enhanced insert expression. Clin Vaccine Immunol. 2011 Jan;18(1):43–49.

Published In

Clin Vaccine Immunol

DOI

EISSN

1556-679X

Publication Date

January 2011

Volume

18

Issue

1

Start / End Page

43 / 49

Location

United States

Related Subject Headings

  • Recombinant Fusion Proteins
  • Rabbits
  • Ovalbumin
  • Mycobacterium bovis
  • Mycobacterium
  • Mutation
  • Mutagenesis, Insertional
  • Microbiology
  • Mice, Inbred C57BL
  • Mice