Generation of CD8+ T-cell responses by a recombinant nonpathogenic Mycobacterium smegmatis vaccine vector expressing human immunodeficiency virus type 1 Env.

Journal Article (Journal Article)

Because the vaccine vectors currently being evaluated in human populations all have significant limitations in their immunogenicity, novel vaccine strategies are needed for the elicitation of cell-mediated immunity. The nonpathogenic, rapidly growing mycobacterium Mycobacterium smegmatis was engineered as a vector expressing full-length human immunodeficiency virus type 1 (HIV-1) HXBc2 envelope protein. Immunization of mice with recombinant M. smegmatis led to the expansion of major histocompatibility complex class I-restricted HIV-1 epitope-specific CD8(+) T cells that were cytolytic and secreted gamma interferon. Effector and memory T lymphocytes were elicited, and repeated immunization generated a stable central memory pool of virus-specific cells. Importantly, preexisting immunity to Mycobacterium bovis BCG had only a marginal effect on the immunogenicity of recombinant M. smegmatis. This mycobacterium may therefore be a useful vaccine vector.

Full Text

Duke Authors

Cited Authors

  • Cayabyab, MJ; Hovav, A-H; Hsu, T; Krivulka, GR; Lifton, MA; Gorgone, DA; Fennelly, GJ; Haynes, BF; Jacobs, WR; Letvin, NL

Published Date

  • February 2006

Published In

Volume / Issue

  • 80 / 4

Start / End Page

  • 1645 - 1652

PubMed ID

  • 16439521

Pubmed Central ID

  • PMC1367151

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/JVI.80.4.1645-1652.2006


  • eng

Conference Location

  • United States