DNA vaccination in rhesus macaques induces potent immune responses and decreases acute and chronic viremia after SIVmac251 challenge.

Published

Journal Article

Optimized plasmid DNAs encoding the majority of SIVmac239 proteins and delivered by electroporation (EP) elicited strong immune responses in rhesus macaques. Vaccination decreased viremia in both the acute and chronic phases of infection after challenge with pathogenic SIVmac251. Two groups of macaques were vaccinated with DNA plasmids producing different antigen forms, "native" and "modified," inducing distinct immune responses. Both groups showed significantly lower viremia during the acute phase of infection, whereas the group immunized with the native antigens showed better protection during the chronic phase (1.7 log decrease in virus load, P = 0.009). Both groups developed strong cellular and humoral responses against the DNA vaccine antigens, which included Gag, Pol, Env, Nef, and Tat. Vaccination induced both central memory and effector memory T cells that were maintained at the day of challenge, suggesting the potential for rapid mobilization upon virus challenge. The group receiving the native antigens developed higher and more durable anti-Env antibodies, including neutralizing antibodies at the day of challenge. These results demonstrate that DNA vaccination in the absence of any heterologous boost can provide protection from high viremia comparable to any other vaccine modalities tested in this macaque model.

Full Text

Duke Authors

Cited Authors

  • Rosati, M; Bergamaschi, C; Valentin, A; Kulkarni, V; Jalah, R; Alicea, C; Patel, V; von Gegerfelt, AS; Montefiori, DC; Venzon, DJ; Khan, AS; Draghia-Akli, R; Van Rompay, KKA; Felber, BK; Pavlakis, GN

Published Date

  • September 15, 2009

Published In

Volume / Issue

  • 106 / 37

Start / End Page

  • 15831 - 15836

PubMed ID

  • 19717425

Pubmed Central ID

  • 19717425

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0902628106

Language

  • eng

Conference Location

  • United States