Role for the target enzyme in deactivation of photoreceptor G protein in vivo.

Journal Article (Journal Article)

Heterotrimeric guanosine 5'-triphosphate (GTP)-binding proteins (G proteins) are deactivated by hydrolysis of the GTP that they bind when activated by transmembrane receptors. Transducin, the G protein that relays visual excitation from rhodopsin to the cyclic guanosine 3',5'-monophosphate phosphodiesterase (PDE) in retinal photoreceptors, must be deactivated for the light response to recover. A point mutation in the gamma subunit of PDE impaired transducin-PDE interactions and slowed the recovery rate of the flash response in transgenic mouse rods. These results indicate that the normal deactivation of transducin in vivo requires the G protein to interact with its target enzyme.

Full Text

Duke Authors

Cited Authors

  • Tsang, SH; Burns, ME; Calvert, PD; Gouras, P; Baylor, DA; Goff, SP; Arshavsky, VY

Published Date

  • October 2, 1998

Published In

Volume / Issue

  • 282 / 5386

Start / End Page

  • 117 - 121

PubMed ID

  • 9756475

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.282.5386.117


  • eng

Conference Location

  • United States