Role for the target enzyme in deactivation of photoreceptor G protein in vivo.
Journal Article (Journal Article)
Heterotrimeric guanosine 5'-triphosphate (GTP)-binding proteins (G proteins) are deactivated by hydrolysis of the GTP that they bind when activated by transmembrane receptors. Transducin, the G protein that relays visual excitation from rhodopsin to the cyclic guanosine 3',5'-monophosphate phosphodiesterase (PDE) in retinal photoreceptors, must be deactivated for the light response to recover. A point mutation in the gamma subunit of PDE impaired transducin-PDE interactions and slowed the recovery rate of the flash response in transgenic mouse rods. These results indicate that the normal deactivation of transducin in vivo requires the G protein to interact with its target enzyme.
Full Text
Duke Authors
Cited Authors
- Tsang, SH; Burns, ME; Calvert, PD; Gouras, P; Baylor, DA; Goff, SP; Arshavsky, VY
Published Date
- October 2, 1998
Published In
Volume / Issue
- 282 / 5386
Start / End Page
- 117 - 121
PubMed ID
- 9756475
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.282.5386.117
Language
- eng
Conference Location
- United States