Population dynamics of ingested Clostridium difficile in the gastrointestinal tract of the Syrian hamster.

Journal Article (Journal Article)

The population dynamics of Clostridium difficile in the hamster gastrointestinal tract were studied after intragastric inoculation with organisms and a 51Cr tracer. Seventy-eight percent of spores germinated within the small intestine within 1 hr. Germinated spores and vegetative cells both showed two phases of elimination from the hamster cecum--an initial phase of rapid death that was not affected by antibiotic treatment followed by a phase of complete inhibition of multiplication. The latter phase of inhibition was not seen in antibiotic-treated animals and was thus attributable to the indigenous flora. The 51Cr tracer mixed well with cecal contents and was eliminated exponentially with a dilution rate constant ranging from -0.46/hr to -0.31/hr in normal hamsters. The hamster cecum was therefore dynamically analogous to a continuous flow system, a finding supporting the concept that anaerobic continuous flow cultures are useful in vitro models of the cecal ecosystem.

Full Text

Duke Authors

Cited Authors

  • Wilson, KH; Sheagren, JN; Freter, R

Published Date

  • February 1985

Published In

Volume / Issue

  • 151 / 2

Start / End Page

  • 355 - 361

PubMed ID

  • 3968453

International Standard Serial Number (ISSN)

  • 0022-1899

Digital Object Identifier (DOI)

  • 10.1093/infdis/151.2.355


  • eng

Conference Location

  • United States