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Cysteine-rich regions of pig gastric mucin contain von willebrand factor and cystine knot domains at the carboxyl terminal(1).

Publication ,  Journal Article
Turner, BS; Bhaskar, KR; Hadzopoulou-Cladaras, M; LaMont, JT
Published in: Biochimica et biophysica acta
October 1999

In order to sequence the cysteine-rich regions of pig gastric mucin (PGM), we used our previously identified pig gastric mucin clone PGM-2A to screen a pig stomach cDNA library and perform rapid amplification of cDNA ends to obtain two cysteine-rich clones, PGM-2X and PGM-Z13. PGM-2X has 1071 base pairs (bp) encoding 357 amino acids containing five serine-threonine-rich 16 amino acid tandem repeats, downstream from a cysteine-rich region similar to human and mouse MUC5AC. PGM-Z13 encodes the complete 3'-terminus of PGM and is composed of 3336 bp with a 2964 bp open reading frame encoding 988 amino acids with four serine-threonine-rich tandem repeats upstream from a cysteine-rich region similar to the carboxyl terminal regions of human and rat MUC5AC and human MUC5B. This region is homologous to von Willebrand factor C and D domains involved in acid induced polymerization, and to the carboxyl terminal cystine-knot domain of various mucins, TGF-beta, vWF and norrin, which is involved in dimerization. These newly sequenced cysteine-rich regions of pig gastric mucin may be critical for its gelation and for its observed increased viscosity induced by low pH.

Duke Scholars

Published In

Biochimica et biophysica acta

DOI

EISSN

1878-2434

ISSN

0006-3002

Publication Date

October 1999

Volume

1447

Issue

1

Start / End Page

77 / 92

Related Subject Headings

  • von Willebrand Factor
  • Threonine
  • Tandem Repeat Sequences
  • Swine
  • Serine
  • Sequence Alignment
  • Molecular Sequence Data
  • Hydrogen-Ion Concentration
  • Gastric Mucosa
  • Gastric Mucins
 

Citation

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MLA
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Turner, B. S., Bhaskar, K. R., Hadzopoulou-Cladaras, M., & LaMont, J. T. (1999). Cysteine-rich regions of pig gastric mucin contain von willebrand factor and cystine knot domains at the carboxyl terminal(1). Biochimica et Biophysica Acta, 1447(1), 77–92. https://doi.org/10.1016/s0167-4781(99)00099-8
Turner, B. S., K. R. Bhaskar, M. Hadzopoulou-Cladaras, and J. T. LaMont. “Cysteine-rich regions of pig gastric mucin contain von willebrand factor and cystine knot domains at the carboxyl terminal(1).Biochimica et Biophysica Acta 1447, no. 1 (October 1999): 77–92. https://doi.org/10.1016/s0167-4781(99)00099-8.
Turner BS, Bhaskar KR, Hadzopoulou-Cladaras M, LaMont JT. Cysteine-rich regions of pig gastric mucin contain von willebrand factor and cystine knot domains at the carboxyl terminal(1). Biochimica et biophysica acta. 1999 Oct;1447(1):77–92.
Turner, B. S., et al. “Cysteine-rich regions of pig gastric mucin contain von willebrand factor and cystine knot domains at the carboxyl terminal(1).Biochimica et Biophysica Acta, vol. 1447, no. 1, Oct. 1999, pp. 77–92. Epmc, doi:10.1016/s0167-4781(99)00099-8.
Turner BS, Bhaskar KR, Hadzopoulou-Cladaras M, LaMont JT. Cysteine-rich regions of pig gastric mucin contain von willebrand factor and cystine knot domains at the carboxyl terminal(1). Biochimica et biophysica acta. 1999 Oct;1447(1):77–92.

Published In

Biochimica et biophysica acta

DOI

EISSN

1878-2434

ISSN

0006-3002

Publication Date

October 1999

Volume

1447

Issue

1

Start / End Page

77 / 92

Related Subject Headings

  • von Willebrand Factor
  • Threonine
  • Tandem Repeat Sequences
  • Swine
  • Serine
  • Sequence Alignment
  • Molecular Sequence Data
  • Hydrogen-Ion Concentration
  • Gastric Mucosa
  • Gastric Mucins