Purification of human renal renin.

Journal Article (Journal Article)

1. Human renal renin has been purified 200 000-fold from cadaver kidney cortex by a method which employs affinity chromatography on aminohexyl peptstatin. 2. The product of this purification has a specific activity of 400 Goldblatt units/mg when compared with Haas human renin standard. 3. This product appears as a single band on sodium dodecyl sulphate gel and polyacrylamide-disc gel electrophoresis. Renin enzymatic activity was recovered after elution from a polyacrylamide-disc gel run at pH 7.8. 4. Yield with this method was 1%.

Full Text

Duke Authors

Cited Authors

  • Slater, EE; Cohn, RC; Dzau, VJ; Haber, E

Published Date

  • December 1, 1978

Published In

Volume / Issue

  • 4 /

Start / End Page

  • 117s - 119s

PubMed ID

  • 282038

International Standard Serial Number (ISSN)

  • 0144-4107

Digital Object Identifier (DOI)

  • 10.1042/cs055117s


  • eng

Conference Location

  • England