Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein.
Journal Article (Journal Article)
The binding of the mutS gene product, a protein involved in at least two E. coli mismatch correction pathways, to a series of synthetic DNA duplexes containing mismatches or mismatch analogues of the purine/pyrimidine type was studied in order to establish whether a correlation exists between the recognition of these mispairs and the efficiency of their correction in vivo. Experiments using nitrocellulose filter binding or band-shift assays revealed that duplexes containing a G/T mismatch or its analogues I/T and DI/T were bound by the protein with affinities correlating to the efficiency of their repair in vivo. In contrast, the A/C mismatch, contained within the same sequence, was bound only poorly, despite being efficiently corrected in vivo. The analogues of the A/C mispair, uncorrected in vivo, were not detectably bound under the conditions of these assays.
Full Text
Duke Authors
Cited Authors
- Jiricny, J; Su, SS; Wood, SG; Modrich, P
Published Date
- August 25, 1988
Published In
Volume / Issue
- 16 / 16
Start / End Page
- 7843 - 7853
PubMed ID
- 3047673
Pubmed Central ID
- PMC338495
International Standard Serial Number (ISSN)
- 0305-1048
Digital Object Identifier (DOI)
- 10.1093/nar/16.16.7843
Language
- eng
Conference Location
- England