Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein.

Published

Journal Article

The binding of the mutS gene product, a protein involved in at least two E. coli mismatch correction pathways, to a series of synthetic DNA duplexes containing mismatches or mismatch analogues of the purine/pyrimidine type was studied in order to establish whether a correlation exists between the recognition of these mispairs and the efficiency of their correction in vivo. Experiments using nitrocellulose filter binding or band-shift assays revealed that duplexes containing a G/T mismatch or its analogues I/T and DI/T were bound by the protein with affinities correlating to the efficiency of their repair in vivo. In contrast, the A/C mismatch, contained within the same sequence, was bound only poorly, despite being efficiently corrected in vivo. The analogues of the A/C mispair, uncorrected in vivo, were not detectably bound under the conditions of these assays.

Full Text

Duke Authors

Cited Authors

  • Jiricny, J; Su, SS; Wood, SG; Modrich, P

Published Date

  • August 25, 1988

Published In

Volume / Issue

  • 16 / 16

Start / End Page

  • 7843 - 7853

PubMed ID

  • 3047673

Pubmed Central ID

  • 3047673

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/16.16.7843

Language

  • eng

Conference Location

  • England