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Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein.

Publication ,  Journal Article
Jiricny, J; Su, SS; Wood, SG; Modrich, P
Published in: Nucleic Acids Res
August 25, 1988

The binding of the mutS gene product, a protein involved in at least two E. coli mismatch correction pathways, to a series of synthetic DNA duplexes containing mismatches or mismatch analogues of the purine/pyrimidine type was studied in order to establish whether a correlation exists between the recognition of these mispairs and the efficiency of their correction in vivo. Experiments using nitrocellulose filter binding or band-shift assays revealed that duplexes containing a G/T mismatch or its analogues I/T and DI/T were bound by the protein with affinities correlating to the efficiency of their repair in vivo. In contrast, the A/C mismatch, contained within the same sequence, was bound only poorly, despite being efficiently corrected in vivo. The analogues of the A/C mispair, uncorrected in vivo, were not detectably bound under the conditions of these assays.

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Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

August 25, 1988

Volume

16

Issue

16

Start / End Page

7843 / 7853

Location

England

Related Subject Headings

  • Pyrophosphatases
  • Phosphoric Monoester Hydrolases
  • Oligonucleotides
  • Nucleic Acid Heteroduplexes
  • Nucleic Acid Conformation
  • Genes, Bacterial
  • Escherichia coli Proteins
  • Escherichia coli
  • Developmental Biology
  • DNA-Binding Proteins
 

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Jiricny, J., Su, S. S., Wood, S. G., & Modrich, P. (1988). Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein. Nucleic Acids Res, 16(16), 7843–7853. https://doi.org/10.1093/nar/16.16.7843
Jiricny, J., S. S. Su, S. G. Wood, and P. Modrich. “Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein.Nucleic Acids Res 16, no. 16 (August 25, 1988): 7843–53. https://doi.org/10.1093/nar/16.16.7843.
Jiricny J, Su SS, Wood SG, Modrich P. Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein. Nucleic Acids Res. 1988 Aug 25;16(16):7843–53.
Jiricny, J., et al. “Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein.Nucleic Acids Res, vol. 16, no. 16, Aug. 1988, pp. 7843–53. Pubmed, doi:10.1093/nar/16.16.7843.
Jiricny J, Su SS, Wood SG, Modrich P. Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein. Nucleic Acids Res. 1988 Aug 25;16(16):7843–7853.
Journal cover image

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

August 25, 1988

Volume

16

Issue

16

Start / End Page

7843 / 7853

Location

England

Related Subject Headings

  • Pyrophosphatases
  • Phosphoric Monoester Hydrolases
  • Oligonucleotides
  • Nucleic Acid Heteroduplexes
  • Nucleic Acid Conformation
  • Genes, Bacterial
  • Escherichia coli Proteins
  • Escherichia coli
  • Developmental Biology
  • DNA-Binding Proteins