Characterization of a trimeric MPER containing HIV-1 gp41 antigen.
Journal Article (Journal Article)
The membrane-proximal external region (MPER) of gp41 is considered as a prime target for the induction of neutralizing antibodies, since it contains the epitopes for three broadly neutralizing antibodies (2F5, 4E10 and Z13). Here we present a novel gp41 construct (HA-gp41) comprising gp41 HR2 and MPER fused to two triple-stranded coiled-coil domains at both ends. HA-gp41 is trimeric, has a high helical content in solution and forms rod-like structures as revealed by negative staining electron microscopy. Immunization of rabbits with HA-gp41 induced antibodies directed against MPER, which failed to exert significant neutralization capacity against envelopes from primary isolates. Thus trimerisation of MPER regions does not suffice to induce a potent neutralizing antibody response specific for conserved regions within gp41.
- Hinz, A; Schoehn, G; Quendler, H; Hulsik, DL; Stiegler, G; Katinger, H; Seaman, MS; Montefiori, D; Weissenhorn, W
- August 1, 2009
Volume / Issue
- 390 / 2
Start / End Page
- 221 - 227
Electronic International Standard Serial Number (EISSN)
Digital Object Identifier (DOI)
- United States