Characterization of a trimeric MPER containing HIV-1 gp41 antigen.

Published

Journal Article

The membrane-proximal external region (MPER) of gp41 is considered as a prime target for the induction of neutralizing antibodies, since it contains the epitopes for three broadly neutralizing antibodies (2F5, 4E10 and Z13). Here we present a novel gp41 construct (HA-gp41) comprising gp41 HR2 and MPER fused to two triple-stranded coiled-coil domains at both ends. HA-gp41 is trimeric, has a high helical content in solution and forms rod-like structures as revealed by negative staining electron microscopy. Immunization of rabbits with HA-gp41 induced antibodies directed against MPER, which failed to exert significant neutralization capacity against envelopes from primary isolates. Thus trimerisation of MPER regions does not suffice to induce a potent neutralizing antibody response specific for conserved regions within gp41.

Full Text

Duke Authors

Cited Authors

  • Hinz, A; Schoehn, G; Quendler, H; Hulsik, DL; Stiegler, G; Katinger, H; Seaman, MS; Montefiori, D; Weissenhorn, W

Published Date

  • August 1, 2009

Published In

Volume / Issue

  • 390 / 2

Start / End Page

  • 221 - 227

PubMed ID

  • 19539967

Pubmed Central ID

  • 19539967

Electronic International Standard Serial Number (EISSN)

  • 1096-0341

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2009.05.015

Language

  • eng

Conference Location

  • United States