Polyvalent DNA prime and envelope protein boost HIV-1 vaccine elicits humoral and cellular responses and controls plasma viremia in rhesus macaques following rectal challenge with an R5 SHIV isolate.

Published

Journal Article

Immunization of macaques with multivalent DNA encoding gp120 genes from HIV-1 subtypes A, B, C and E and a gag gene followed by boosting with homologous gp120 proteins elicited strong anti-gp120 antibodies capable of neutralizing homologous and to a lesser degree heterologous HIV-1 isolates. Both Env- and Gag-specific cell mediated immune (CMI) responses were detected in the immunized animals. Following rectal challenge with an SHIV isolate encoding HIV-1(Ba-L)env, plasma viremia in the infected immunized animals was significantly lower than that observed in the naïve animals. Further, one of six immunized animals was completely protected whereas all six naïve animals were infected. These results demonstrate that a vaccine based on priming with a polyvalent DNA vaccine from multiple HIV-1 subtypes followed by boosting with homologous Env proteins elicits anti-HIV-1 immune responses capable of controlling rectal transmission of SHIV(Ba-L).

Full Text

Duke Authors

Cited Authors

  • Pal, R; Wang, S; Kalyanaraman, VS; Nair, BC; Whitney, S; Keen, T; Hocker, L; Hudacik, L; Rose, N; Cristillo, A; Mboudjeka, I; Shen, S; Wu-Chou, T-H; Montefiori, D; Mascola, J; Lu, S; Markham, P

Published Date

  • October 2005

Published In

Volume / Issue

  • 34 / 5-6

Start / End Page

  • 226 - 236

PubMed ID

  • 16128917

Pubmed Central ID

  • 16128917

International Standard Serial Number (ISSN)

  • 0047-2565

Digital Object Identifier (DOI)

  • 10.1111/j.1600-0684.2005.00120.x

Language

  • eng

Conference Location

  • Denmark