Fiber optic fluorescence immunoassay: use of a biotin-avidin bridge as a generic antibody immobilization technique

In this study a fluorescence immunoassay is performed using a biotin-avidin bridge was used to immobilize biotinylated antibodies to the surface of a silanized fiber optic. The surface chemistry is verified using TIRF (Total Internal Reflectance Fluorescence) and with a fiber optic assembly, which consisting of two fibers joined by a Y-type coupler, The antibody binding sensitivity of the fiber optic configuration is 10-9 M concentration, with a reduced antigen binding sensitivity of 10-6 M concentration. Future studies will examine the fluorescence signal decay due to the immobilization technique and manipulation of antibody paratope orientation by means of alternative surface chemistries.

Duke Scholars

Author William M. Reichert Biomedical Engineering