Fiber optic fluorescence immunoassay: use of a biotin-avidin bridge as a generic antibody immobilization technique


Journal Article

In this study a fluorescence immunoassay is performed using a biotin-avidin bridge was used to immobilize biotinylated antibodies to the surface of a silanized fiber optic. The surface chemistry is verified using TIRF (Total Internal Reflectance Fluorescence) and with a fiber optic assembly, which consisting of two fibers joined by a Y-type coupler, The antibody binding sensitivity of the fiber optic configuration is 10-9 M concentration, with a reduced antigen binding sensitivity of 10-6 M concentration. Future studies will examine the fluorescence signal decay due to the immobilization technique and manipulation of antibody paratope orientation by means of alternative surface chemistries.

Duke Authors

Cited Authors

  • Savage, AL; Reichert, WM

Published Date

  • December 1, 1993

Published In

Volume / Issue

  • 15 / pt 3

Start / End Page

  • 1547 - 1548

International Standard Serial Number (ISSN)

  • 0589-1019

Citation Source

  • Scopus