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Fiber optic fluorescence immunoassay: use of a biotin-avidin bridge as a generic antibody immobilization technique

Publication ,  Journal Article
Savage, AL; Reichert, WM
Published in: Proceedings of the Annual Conference on Engineering in Medicine and Biology
December 1, 1993

In this study a fluorescence immunoassay is performed using a biotin-avidin bridge was used to immobilize biotinylated antibodies to the surface of a silanized fiber optic. The surface chemistry is verified using TIRF (Total Internal Reflectance Fluorescence) and with a fiber optic assembly, which consisting of two fibers joined by a Y-type coupler, The antibody binding sensitivity of the fiber optic configuration is 10-9 M concentration, with a reduced antigen binding sensitivity of 10-6 M concentration. Future studies will examine the fluorescence signal decay due to the immobilization technique and manipulation of antibody paratope orientation by means of alternative surface chemistries.

Duke Scholars

Published In

Proceedings of the Annual Conference on Engineering in Medicine and Biology

ISSN

0589-1019

Publication Date

December 1, 1993

Volume

15

Issue

pt 3

Start / End Page

1547 / 1548
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Savage, A. L., & Reichert, W. M. (1993). Fiber optic fluorescence immunoassay: use of a biotin-avidin bridge as a generic antibody immobilization technique. Proceedings of the Annual Conference on Engineering in Medicine and Biology, 15(pt 3), 1547–1548.
Savage, A. L., and W. M. Reichert. “Fiber optic fluorescence immunoassay: use of a biotin-avidin bridge as a generic antibody immobilization technique.” Proceedings of the Annual Conference on Engineering in Medicine and Biology 15, no. pt 3 (December 1, 1993): 1547–48.
Savage AL, Reichert WM. Fiber optic fluorescence immunoassay: use of a biotin-avidin bridge as a generic antibody immobilization technique. Proceedings of the Annual Conference on Engineering in Medicine and Biology. 1993 Dec 1;15(pt 3):1547–8.
Savage, A. L., and W. M. Reichert. “Fiber optic fluorescence immunoassay: use of a biotin-avidin bridge as a generic antibody immobilization technique.” Proceedings of the Annual Conference on Engineering in Medicine and Biology, vol. 15, no. pt 3, Dec. 1993, pp. 1547–48.
Savage AL, Reichert WM. Fiber optic fluorescence immunoassay: use of a biotin-avidin bridge as a generic antibody immobilization technique. Proceedings of the Annual Conference on Engineering in Medicine and Biology. 1993 Dec 1;15(pt 3):1547–1548.

Published In

Proceedings of the Annual Conference on Engineering in Medicine and Biology

ISSN

0589-1019

Publication Date

December 1, 1993

Volume

15

Issue

pt 3

Start / End Page

1547 / 1548