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Acrylamide-regulated neurofilament expression in rat pheochromocytoma cells.

Publication ,  Journal Article
Lin, WW; Friedman, MA; Wang, XF; Abou-Donia, MB
Published in: Brain Res
January 10, 2000

Using the rat pheochromocytoma cell line (PC12), we present molecular evidence that the neurotoxicant acrylamide directly induces neurofilament gene expression, and the signaling pathways are initially distinctive from, but eventually merged into, that for nerve growth factor (NGF)-induced neurofilament expression. In PC12 cells, acrylamide increased neurofilament protein levels and synthesis. Acrylamide had no effect on the stability of neurofilament mRNAs suggesting that it directly increased neurofilament mRNA synthesis. K252a, a selective inhibitor for NGF receptor gp140trk, had no effect on acrylamide induction, but completely inhibited NGF-induced neurofilament protein synthesis. Therefore, the initial step for acrylamide signaling was distinctive from NGF. Dexamethasone reversed the effects of both NGF and acrylamide on neurofilament protein levels and synthesis indicated that there is a dexamethasone-sensitive signaling step upon which NGF and acrylamide merge, suggesting involvement of transcription-activating proteins like AP-1. These results, taken together with previous studies of transgenic mice that overexpress neurofilament genes, may partially explain the mechanisms of neurofilament accumulation in distal parts of large axons, a pathognomonic feature of acrylamide neurotoxicity in animals.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

January 10, 2000

Volume

852

Issue

2

Start / End Page

297 / 304

Location

Netherlands

Related Subject Headings

  • Signal Transduction
  • Ribonucleases
  • Receptor, trkA
  • Rats
  • RNA, Messenger
  • PC12 Cells
  • Neurotoxins
  • Neurons
  • Neurology & Neurosurgery
  • Neurofilament Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lin, W. W., Friedman, M. A., Wang, X. F., & Abou-Donia, M. B. (2000). Acrylamide-regulated neurofilament expression in rat pheochromocytoma cells. Brain Res, 852(2), 297–304. https://doi.org/10.1016/s0006-8993(99)02104-6
Lin, W. W., M. A. Friedman, X. F. Wang, and M. B. Abou-Donia. “Acrylamide-regulated neurofilament expression in rat pheochromocytoma cells.Brain Res 852, no. 2 (January 10, 2000): 297–304. https://doi.org/10.1016/s0006-8993(99)02104-6.
Lin WW, Friedman MA, Wang XF, Abou-Donia MB. Acrylamide-regulated neurofilament expression in rat pheochromocytoma cells. Brain Res. 2000 Jan 10;852(2):297–304.
Lin, W. W., et al. “Acrylamide-regulated neurofilament expression in rat pheochromocytoma cells.Brain Res, vol. 852, no. 2, Jan. 2000, pp. 297–304. Pubmed, doi:10.1016/s0006-8993(99)02104-6.
Lin WW, Friedman MA, Wang XF, Abou-Donia MB. Acrylamide-regulated neurofilament expression in rat pheochromocytoma cells. Brain Res. 2000 Jan 10;852(2):297–304.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

January 10, 2000

Volume

852

Issue

2

Start / End Page

297 / 304

Location

Netherlands

Related Subject Headings

  • Signal Transduction
  • Ribonucleases
  • Receptor, trkA
  • Rats
  • RNA, Messenger
  • PC12 Cells
  • Neurotoxins
  • Neurons
  • Neurology & Neurosurgery
  • Neurofilament Proteins