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Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes.

Publication ,  Journal Article
Tietz, PS; Marinelli, RA; Chen, X-M; Huang, B; Cohn, J; Kole, J; McNiven, MA; Alper, S; LaRusso, NF
Published in: J Biol Chem
May 30, 2003

We previously proposed that ductal bile formation is regulated by secretin-responsive relocation of aquaporin 1 (AQP1), a water-selective channel protein, from an intracellular vesicular compartment to the apical membrane of cholangiocytes. In this study, we immunoisolated AQP1-containing vesicles from cholangiocytes prepared from rat liver; quantitative immunoblotting revealed enrichment in these vesicles of not only AQP1 but also cystic fibrosis transmembrane regulator (CFTR) and AE2, a Cl- channel and a Cl-/HCO3- exchanger, respectively. Dual labeled immunogold electron microscopy of cultured polarized mouse cholangiocytes showed significant colocalization of AQP1, CFTR, and AE2 in an intracellular vesicular compartment; exposure of cholangiocytes to dibutyryl-cAMP (100 microm) resulted in co-redistribution of all three proteins to the apical cholangiocyte plasma membrane. After administration of secretin to rats in vivo, bile flow increased, and AQP1, CFTR, and AE2 co-redistributed to the apical cholangiocyte membrane; both events were blocked by pharmacologic disassembly of microtubules. Based on these in vitro and in vivo observations utilizing independent and complementary approaches, we propose that cholangiocytes contain an organelle that sequesters functionally related proteins that can account for ion-driven water transport, that this organelle moves to the apical cholangiocyte membrane in response to secretory agonists, and that these events account for ductal bile secretion at a molecular level.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 30, 2003

Volume

278

Issue

22

Start / End Page

20413 / 20419

Location

United States

Related Subject Headings

  • Water
  • Secretin
  • SLC4A Proteins
  • Rats, Inbred F344
  • Rats
  • Protein Transport
  • Microscopy, Electron
  • Membrane Proteins
  • Male
  • Ion Transport
 

Citation

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Chicago
ICMJE
MLA
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Tietz, P. S., Marinelli, R. A., Chen, X.-M., Huang, B., Cohn, J., Kole, J., … LaRusso, N. F. (2003). Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes. J Biol Chem, 278(22), 20413–20419. https://doi.org/10.1074/jbc.M302108200
Tietz, Pamela S., Raul A. Marinelli, Xian-Ming Chen, Bing Huang, Jonathan Cohn, Jolanta Kole, Mark A. McNiven, Seth Alper, and Nicholas F. LaRusso. “Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes.J Biol Chem 278, no. 22 (May 30, 2003): 20413–19. https://doi.org/10.1074/jbc.M302108200.
Tietz PS, Marinelli RA, Chen X-M, Huang B, Cohn J, Kole J, et al. Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes. J Biol Chem. 2003 May 30;278(22):20413–9.
Tietz, Pamela S., et al. “Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes.J Biol Chem, vol. 278, no. 22, May 2003, pp. 20413–19. Pubmed, doi:10.1074/jbc.M302108200.
Tietz PS, Marinelli RA, Chen X-M, Huang B, Cohn J, Kole J, McNiven MA, Alper S, LaRusso NF. Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes. J Biol Chem. 2003 May 30;278(22):20413–20419.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 30, 2003

Volume

278

Issue

22

Start / End Page

20413 / 20419

Location

United States

Related Subject Headings

  • Water
  • Secretin
  • SLC4A Proteins
  • Rats, Inbred F344
  • Rats
  • Protein Transport
  • Microscopy, Electron
  • Membrane Proteins
  • Male
  • Ion Transport