Jonathan Allen Cohn | Scholars@Duke

Jonathan Allen Cohn
Professor of Medicine

In many epithelial tissues, the protein mainly responsible for controlling transepithelial fluid movement is the the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR was originally identified as the protein product of the gene causing cystic fibrosis (CF). CFTR functions as a chloride channel regulated by protein kinase A (PKA). This laboratory is studying the role and regulation of CFTR. One project focuses on how PKA acts on CFTR. In this project, recombinant peptide models are being used to model the cytoplasmic domains of CFTR responsible for activating the protein's chloride's chloride channel function. Site-directed mutagenesis is being used to produce modified peptides to study how different individual serine phosphoryation contribute to CFTR regulation. A second project concerns DF508, the most common mutation among patients with CF. This mutation affects CFTR function by preventing normal folding and intracellular trafficking of the newly synthesized mutant protein. This project is examining the mislocalization of DF508-CFTR in tissues and cell lines with the long term goal of developing strategies to prevent the mutant protein from mislocalizing. A third project concerns the role of CFTR in chronic pancreatitic diseases. Emerging data about the role of CFTR during normal pancreatic secretion suggests that dysfunction of this protein may lead to pancreatic diseases such as chronic pancreatitis and pancreatic cancer. Patients with these chronic pancreatic diseases are being tested for CFTR mutations and for evidence of defective CFTR function.

Current Appointments & Affiliations

Professor of Medicine, Medicine, Gastroenterology, Medicine 2002
Associate Professor of Cell Biology, Cell Biology, Basic Science Departments 1996
Professor in Pediatrics, Pediatrics, Pulmonary and Sleep Medicine, Pediatrics 2008

Contact Information

DUMC Box 3256, Durham, NC 27710
GSRB-1 Room 2038, 905 S Lasalle St, Durham, NC 27710
jonathan.cohn@duke.edu (919) 684-6879

Background
Education, Training, & Certifications
- Fellow in Gastroenterology, Medicine, Yale University 1981 - 1984
- Medical Resident, Medicine, University of California San Francisco, School of Medicine 1980 - 1981
- Medical Resident, Medicine, University of Alabama Birmingham 1978 - 1980
- M.D., Cornell University 1978
Previous Appointments & Affiliations
- Associate Professor of Medicine with Tenure, Medicine, Gastroenterology, Medicine 1994 - 2002
- Assistant Professor of Cell Biology, Cell Biology, Basic Science Departments 1994 - 1996
- Assistant Professor of Medicine, Medicine, Gastroenterology, Medicine 1988 - 1994
Research
Selected Grants
- The Duke Multidisciplinary Training Program in Pediatric Lung Disease awarded by National Institutes of Health 2010 - 2016
- The Cystic Fibrosis Gene and Chronic Pancreatitis awarded by National Institutes of Health 2005 - 2012
- Maturation of Normal & Sensitized Airway Contractility awarded by National Institutes of Health 2006 - 2011
- The molecular basis of secretory diarrhea awarded by National Institutes of Health 1999 - 2005
- The Molecular Basis Of Secretory awarded by National Institutes of Health 1999 - 2003
- Secretory Diarrhea: Cellular Mechanisms awarded by National Institutes of Health 1995 - 1997
- Secretory Diarrhea: Cellular Mechanisms awarded by National Institutes of Health 1993 - 1997
- Secretory Diarrhea--Cellular Mechanisms awarded by National Institutes of Health 1988 - 1997
- Secretory Diarrhea: Cellular Mechanisms awarded by National Institutes of Health 1988 - 1997
Publications & Artistic Works
Selected Publications
- Academic Articles
  - Cohn, J. A. “Hereditary Diseases of the Pancreas” 2 (February 17, 2009): 1921–39. https://doi.org/10.1002/9781444303254.ch72.
    Full Text
  - Whitcomb, D. C., and J. A. Cohn. “Pancreatic exocrine dysfunction,” December 1, 2006, 573–80. https://doi.org/10.1007/978-1-59259-963-9_55.
    Full Text
  - Cohn, Jonathan A., John P. Neoptolemos, Jinong Feng, Jin Yan, Zefei Jiang, William Greenhalf, Christopher McFaul, Roger Mountford, and Steve S. Sommer. “Increased risk of idiopathic chronic pancreatitis in cystic fibrosis carriers.” Hum Mutat 26, no. 4 (October 2005): 303–7. https://doi.org/10.1002/humu.20232.
    Full Text Link to Item
  - Cohn, Jonathan A. “A word of thanks.” Gastroenterology 129, no. 3 (September 2005): 784. https://doi.org/10.1053/j.gastro.2005.07.052.
    Full Text Link to Item
  - Cohn, Jonathan A. “Reduced CFTR function and the pathobiology of idiopathic pancreatitis.” J Clin Gastroenterol 39, no. 4 Suppl 2 (April 2005): S70–77. https://doi.org/10.1097/01.mcg.0000155522.89005.bf.
    Full Text Link to Item
  - Cohn, Jonathan A., R Michael Mitchell, and Paul S. Jowell. “The impact of cystic fibrosis and PSTI/SPINK1 gene mutations on susceptibility to chronic pancreatitis.” Clin Lab Med 25, no. 1 (March 2005): 79–100. https://doi.org/10.1016/j.cll.2004.12.007.
    Full Text Link to Item
  - Cohn, Jonathan A., R Michael Mitchell, and Paul S. Jowell. “The role of cystic fibrosis gene mutations in determining susceptibility to chronic pancreatitis.” Gastroenterol Clin North Am 33, no. 4 (December 2004): 817–vii. https://doi.org/10.1016/j.gtc.2004.07.008.
    Full Text Link to Item
  - Cohn, Jonathan A. “An argument for identifying highly cited papers on biographical sketches.” Gastroenterology 127, no. 1 (July 2004): 7. https://doi.org/10.1053/j.gastro.2004.06.012.
    Full Text Link to Item
  - Howell, L Daniel, Roy Borchardt, Jolanta Kole, Andrew M. Kaz, Christoph Randak, and Jonathan A. Cohn. “Protein kinase A regulates ATP hydrolysis and dimerization by a CFTR (cystic fibrosis transmembrane conductance regulator) domain.” Biochem J 378, no. Pt 1 (February 15, 2004): 151–59. https://doi.org/10.1042/BJ20021428.
    Full Text Link to Item
  - Mansfield, S Gary, Rebecca Hawkins Clark, M. Puttaraju, Jolanta Kole, Jonathan A. Cohn, Lloyd G. Mitchell, and Mariano A. Garcia-Blanco. “5' exon replacement and repair by spliceosome-mediated RNA trans-splicing.” Rna 9, no. 10 (October 2003): 1290–97. https://doi.org/10.1261/rna.5101903.
    Full Text Link to Item
  - Tietz, Pamela S., Raul A. Marinelli, Xian-Ming Chen, Bing Huang, Jonathan Cohn, Jolanta Kole, Mark A. McNiven, Seth Alper, and Nicholas F. LaRusso. “Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes.” J Biol Chem 278, no. 22 (May 30, 2003): 20413–19. https://doi.org/10.1074/jbc.M302108200.
    Full Text Link to Item
  - Cohn, Jonathan A. “Lessons from Mendel about data analysis.” Gastroenterology 124, no. 4 (April 2003): 878. https://doi.org/10.1053/gast.2003.50203.
    Full Text Link to Item
  - Cohn, Jonathan A. “Motion--genetic testing is useful in the diagnosis of nonhereditary pancreatic conditions: arguments against the motion.” Can J Gastroenterol 17, no. 1 (January 2003): 53–55. https://doi.org/10.1155/2003/169280.
    Full Text Link to Item
  - Cohn, Jonathan A., Peadar G. Noone, and Paul S. Jowell. “Idiopathic pancreatitis related to CFTR: complex inheritance and identification of a modifier gene.” J Investig Med 50, no. 5 (September 2002): 247S-255S. https://doi.org/10.1136/jim-50-suppl5-01.
    Full Text Link to Item
  - Cohn, Jonathan A. “Putting the spotlight on our most important discoveries.” Gastroenterology 122, no. 2 (February 2002): 257. https://doi.org/10.1053/gast.2002.31737.
    Full Text Link to Item
  - Liu, Xiaoming, Qinshi Jiang, S Gary Mansfield, M. Puttaraju, Yulong Zhang, Weihong Zhou, Jonathan A. Cohn, Mariano A. Garcia-Blanco, Lloyd G. Mitchell, and John F. Engelhardt. “Partial correction of endogenous DeltaF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing.” Nat Biotechnol 20, no. 1 (January 2002): 47–52. https://doi.org/10.1038/nbt0102-47.
    Full Text Link to Item
  - Noone, P. G., Z. Zhou, L. M. Silverman, P. S. Jowell, M. R. Knowles, and J. A. Cohn. “Cystic fibrosis gene mutations and pancreatitis risk: relation to epithelial ion transport and trypsin inhibitor gene mutations.” Gastroenterology 121, no. 6 (December 2001): 1310–19. https://doi.org/10.1053/gast.2001.29673.
    Full Text Link to Item
  - Dérand, R., L. Bulteau-Pignoux, Y. Mettey, O. Zegarra-Moran, L. D. Howell, C. Randak, L. J. Galietta, et al. “Activation of G551D CFTR channel with MPB-91: regulation by ATPase activity and phosphorylation.” Am J Physiol Cell Physiol 281, no. 5 (November 2001): C1657–66. https://doi.org/10.1152/ajpcell.2001.281.5.C1657.
    Full Text Link to Item
  - Boujaoude, L. C., C. Bradshaw-Wilder, C. Mao, J. Cohn, B. Ogretmen, Y. A. Hannun, and L. M. Obeid. “Cystic fibrosis transmembrane regulator regulates uptake of sphingoid base phosphates and lysophosphatidic acid: modulation of cellular activity of sphingosine 1-phosphate.” J Biol Chem 276, no. 38 (September 21, 2001): 35258–64. https://doi.org/10.1074/jbc.M105442200.
    Full Text Link to Item
  - Blaug, S., K. Hybiske, J. Cohn, G. L. Firestone, T. E. Machen, and S. S. Miller. “ENaC- and CFTR-dependent ion and fluid transport in mammary epithelia.” Am J Physiol Cell Physiol 281, no. 2 (August 2001): C633–48. https://doi.org/10.1152/ajpcell.2001.281.2.C633.
    Full Text Link to Item
  - Howell, L Daniel, Melissa E. Danko, Roy Borchardt, Christoph Randak, and Jonathan A. Cohn. “Domain-domain interactions regulate ATP hydrolysis during CFTR activation.” Gastroenterology 120, no. 5 (April 2001): A84–A84. https://doi.org/10.1016/s0016-5085(08)80416-7.
    Full Text
  - Kole, Jolanta, S. Gary Mansfield, M. Puttaraju, Mariano A. Garcia-Blanco, Lloyd G. Mitchell, and Jonathan A. Cohn. “Production of functional CFTR from mutant CFTR mRNA repaired by targeted RNA trans-splicing.” Gastroenterology 120, no. 5 (April 2001): A24–A24. https://doi.org/10.1016/s0016-5085(08)80118-7.
    Full Text
  - Cohn, J. A. “The diagnosis of CF in patients with non-classical phenotypes.” Pediatric Pulmonology 30, no. SUPPL. 20 (December 1, 2000): 89–90.
  - Mansfield, S. G., J. Kole, M. Puttaraju, C. C. Yang, M. A. Garcia-Blanco, J. A. Cohn, and L. G. Mitchell. “Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing.” Gene Ther 7, no. 22 (November 2000): 1885–95. https://doi.org/10.1038/sj.gt.3301307.
    Full Text Link to Item
  - Howell, L. D., R. Borchardt, and J. A. Cohn. “ATP hydrolysis by a CFTR domain: pharmacology and effects of G551D mutation.” Biochem Biophys Res Commun 271, no. 2 (May 10, 2000): 518–25. https://doi.org/10.1006/bbrc.2000.2659.
    Full Text Link to Item
  - Cohn, J. A., J. D. Bornstein, and P. S. Jowell. “Cystic fibrosis mutations and genetic predisposition to idiopathic chronic pancreatitis.” Med Clin North Am 84, no. 3 (May 2000): 621–ix. https://doi.org/10.1016/s0025-7125(05)70245-5.
    Full Text Link to Item
  - Cohn, Jonathan A., Jeffrey D. Bomstein, Paul S. Jowell, Peadar G. Noone, Zhaoqing Zhou, M. S. Branch, John Baillie, William R. Treem, Michael R. Knowles, and Lawrence M. Silverman. “Molecular pathogenesis of chronic pancreatitis associated with abnormal CFTR genotypes.” Gastroenterology 118, no. 4 (April 2000): A159–A159. https://doi.org/10.1016/s0016-5085(00)82713-4.
    Full Text
  - Whitcomb, D. C., J. A. Cohn, and C. D. Ulrich. “Inherited diseases of the pancreas: Preface.” Medical Clinics of North America 84, no. 3 (January 1, 2000). https://doi.org/10.1016/s0025-7125(05)70236-4.
    Full Text
  - Friedman, K. J., J. Kole, J. A. Cohn, M. R. Knowles, L. M. Silverman, and R. Kole. “Correction of aberrant splicing of the cystic fibrosis transmembrane conductance regulator (CFTR) gene by antisense oligonucleotides.” J Biol Chem 274, no. 51 (December 17, 1999): 36193–99. https://doi.org/10.1074/jbc.274.51.36193.
    Full Text Link to Item
  - Cohn, J. A. “Is idiopathic chronic pancreatitis a form of cystic fibrosis?” Pediatric Pulmonology 28, no. SUPPL. 19 (September 1, 1999): 91.
  - Cohn, J. A., Z. Zhou, J. D. Bornstein, K. J. Friedman, P. S. Jowell, S. M. Branch, J. Baillie, L. M. Silverman, M. R. Knowles, and P. G. Noone. “CFTR mutations and idiopathic chronic pancreatitis.” Pediatric Pulmonology 28, no. SUPPL. 19 (September 1, 1999): 130–31.
  - Cohn, J. A., and P. S. Jowell. “Are mutations in the cystic fibrosis gene important in chronic pancreatitis?” Surg Clin North Am 79, no. 4 (August 1999): 723–viii. https://doi.org/10.1016/s0039-6109(05)70038-4.
    Full Text Link to Item
  - Bornstein, J. D., and J. A. Cohn. “Cystic fibrosis in the pancreas: recent advances provide new insights.” Curr Gastroenterol Rep 1, no. 2 (April 1999): 161–65. https://doi.org/10.1007/s11894-996-0016-6.
    Full Text Link to Item
  - Cohn, J. A., L. M. Silverman, and M. R. Knowles. “Mutations of the cystic fibrosis gene and pancreatitis - Reply.” New England Journal of Medicine 340, no. 3 (January 21, 1999): 239–239.
    Link to Item
  - Ren, C. L. “Mutations of the cystic fibrosis gene and pancreatitis.” N Engl J Med 340, no. 3 (January 21, 1999): 238–39. https://doi.org/10.1056/NEJM199901213400316.
    Full Text Link to Item
  - Cohn, J. A., K. J. Friedman, P. G. Noone, M. R. Knowles, L. M. Silverman, and P. S. Jowell. “Relation between mutations of the cystic fibrosis gene and idiopathic pancreatitis.” N Engl J Med 339, no. 10 (September 3, 1998): 653–58. https://doi.org/10.1056/NEJM199809033391002.
    Full Text Link to Item
  - Cohn, J. A., K. J. Friedman, L. M. Silverman, P. G. Noone, C. Q. Lee, M. R. Knowles, and P. S. Jowell. “Abnormalities of the CFTR gene predispose to idiopathic chronic pancreatitis.” Gastroenterology 114 (April 1998): A449–A449. https://doi.org/10.1016/s0016-5085(98)81815-5.
    Full Text
  - Howell, L. D., L. Chien, and J. A. Cohn. “Protein kinase a regulates ATP hydrolysis by the CFTR NBD1/R domain.” Gastroenterology 114 (April 1998): A379–A379. https://doi.org/10.1016/s0016-5085(98)81536-9.
    Full Text
  - Xiong, X., A. Bragin, J. H. Widdicombe, J. Cohn, and W. R. Skach. “Structural cues involved in endoplasmic reticulum degradation of G85E and G91R mutant cystic fibrosis transmembrane conductance regulator.” J Clin Invest 100, no. 5 (September 1, 1997): 1079–88. https://doi.org/10.1172/JCI119618.
    Full Text Link to Item
  - Abdullah, L. H., J. D. Conway, J. A. Cohn, and C. W. Davis. “Protein kinase C and Ca2+ activation of mucin secretion in airway goblet cells.” Am J Physiol 273, no. 1 Pt 1 (July 1997): L201–10. https://doi.org/10.1152/ajplung.1997.273.1.L201.
    Full Text Link to Item
  - Abdullah, L. H., J. D. Conway, J. A. Cohn, and C. W. Davis. “Protein kinase C and Ca2+ activation of mucin secretion in airway goblet cells.” American Journal of Physiology Lung Cellular and Molecular Physiology 273, no. 1 (July 1, 1997): L201–10.
    Link to Item
  - Montrose-Rafizadeh, C., J. Kole, L. M. Bartkowski, L. H. Lee, D. L. Blackmon, S. E. Behnken, J. D. Gearhart, J. A. Cohn, and M. H. Montrose. “Gene targeting of a CFTR allele in HT29 human epithelial cells.” J Cell Physiol 170, no. 3 (March 1997): 299–308. https://doi.org/10.1002/(SICI)1097-4652(199703)170:3<299::AID-JCP11>3.0.CO;2-A.
    Full Text Link to Item
  - Torres, R. J., G. A. Altenberg, J. Cohn, and L. Reuss. “Polarized apical-membrane expression of cAMP-actlvated chloride channels in isolated epithelial cells.” Faseb Journal 10, no. 3 (December 1, 1996).
  - Torres, R. J., G. A. Altenberg, J. A. Cohn, and L. Reuss. “Polarized expression of cAMP-activated chloride channels in isolated epithelial cells.” Am J Physiol 271, no. 5 Pt 1 (November 1996): C1574–82. https://doi.org/10.1152/ajpcell.1996.271.5.C1574.
    Full Text Link to Item
  - Torres, R. J., G. A. Altenberg, J. A. Cohn, and L. Reuss. “Polarized expression of cAMP-activated chloride channels in isolated epithelial cells.” American Journal of Physiology Cell Physiology 271, no. 5 (November 1, 1996): C1574–82.
    Link to Item
  - Stutts, M. J., C. M. Canessa, J. C. Olsen, M. Hamrick, J. A. Cohn, B. C. Rossier, and R. C. Boucher. “CFTR as a cAMP-dependent regulator of sodium channels.” Science 269, no. 5225 (August 11, 1995): 847–50. https://doi.org/10.1126/science.7543698.
    Full Text Link to Item
  - Chen, J., J. A. Cohn, and L. J. Mandel. “Dephosphorylation of ezrin as an early event in renal microvillar breakdown and anoxic injury.” Proc Natl Acad Sci U S A 92, no. 16 (August 1, 1995): 7495–99. https://doi.org/10.1073/pnas.92.16.7495.
    Full Text Link to Item
  - Borchardt, R. A., A. M. Kaz, J. Kole, and J. A. Cohn. “Protein kinase A and protein kinase C phosphorylate distinct serine residues in CFTR.” Gastroenterology 108, no. 4 (April 1995): A275–A275. https://doi.org/10.1016/0016-5085(95)23760-7.
    Full Text
  - Smit, L. S., T. V. Strong, D. J. Wilkinson, M. Macek, M. K. Mansoura, D. L. Wood, J. L. Cole, G. R. Cutting, J. A. Cohn, and D. C. Dawson. “Missense mutation (G480C) in the CFTR gene associated with protein mislocalization but normal chloride channel activity.” Hum Mol Genet 4, no. 2 (February 1995): 269–73. https://doi.org/10.1093/hmg/4.2.269.
    Full Text Link to Item
  - ERNST, S. A., K. M. CRAWFORD, M. A. POST, and J. A. COHN. “SALT STRESS INCREASES ABUNDANCE AND GLYCOSYLATION OF CFTR LOCALIZED AT APICAL SURFACES OF SALT-GLAND SECRETORY-CELLS.” American Journal of Physiology Cell Physiology 267, no. 4 (October 1, 1994): C990–1001.
    Link to Item
  - Ernst, S. A., K. M. Crawford, M. A. Post, and J. A. Cohn. “Salt stress increases abundance and glycosylation of CFTR localized at apical surfaces of salt gland secretory cells.” Am J Physiol 267, no. 4 Pt 1 (October 1994): C990-1001. https://doi.org/10.1152/ajpcell.1994.267.4.C990.
    Full Text Link to Item
  - Bradbury, N. A., J. A. Cohn, C. J. Venglarik, and R. J. Bridges. “Biochemical and biophysical identification of cystic fibrosis transmembrane conductance regulator chloride channels as components of endocytic clathrin-coated vesicles.” J Biol Chem 269, no. 11 (March 18, 1994): 8296–8302.
    Link to Item
  - Engelhardt, J. F., M. Zepeda, J. A. Cohn, J. R. Yankaskas, and J. M. Wilson. “Expression of the cystic fibrosis gene in adult human lung.” J Clin Invest 93, no. 2 (February 1994): 737–49. https://doi.org/10.1172/JCI117028.
    Full Text Link to Item
  - Ernst, S. A., K. M. Crawford, M. A. Post, and J. A. Cohn. “Salt stress increases abundance and glycosylation of CFTR localized at apical surfaces of salt gland secretory cells.” American Journal of Physiology Cell Physiology 267, no. 4 37-4 (January 1, 1994).
  - Cohn, J. A., T. V. Strong, M. R. Picciotto, A. C. Nairn, F. S. Collins, and J. G. Fitz. “Localization of the cystic fibrosis transmembrane conductance regulator in human bile duct epithelial cells.” Gastroenterology 105, no. 6 (December 1993): 1857–64. https://doi.org/10.1016/0016-5085(93)91085-v.
    Full Text Link to Item
  - Yang, Y., S. Janich, J. A. Cohn, and J. M. Wilson. “The common variant of cystic fibrosis transmembrane conductance regulator is recognized by hsp70 and degraded in a pre-Golgi nonlysosomal compartment.” Proc Natl Acad Sci U S A 90, no. 20 (October 15, 1993): 9480–84. https://doi.org/10.1073/pnas.90.20.9480.
    Full Text Link to Item
  - Cohn, J. A., J. Kole, and J. R. Yankaskas. “Protein phosphorylation responses in normal and cystic fibrosis airway epithelial cell lines.” Am J Respir Cell Mol Biol 9, no. 4 (October 1993): 401–4. https://doi.org/10.1165/ajrcmb/9.4.401.
    Full Text Link to Item
  - Yang, Y., D. C. Devor, J. F. Engelhardt, S. A. Ernst, T. V. Strong, F. S. Collins, J. A. Cohn, R. A. Frizzell, and J. M. Wilson. “Molecular basis of defective anion transport in L cells expressing recombinant forms of CFTR.” Hum Mol Genet 2, no. 8 (August 1993): 1253–61. https://doi.org/10.1093/hmg/2.8.1253.
    Full Text Link to Item
  - Basavappa, S., J. Middleton, A. W. Mangel, J. M. McGill, J. A. Cohn, and J. G. Fitz. “Cl- and K+ transport in human biliary cell lines.” Gastroenterology 104, no. 6 (June 1993): 1796–1805. https://doi.org/10.1016/0016-5085(93)90661-u.
    Full Text Link to Item
  - Yang, Y., S. E. Raper, J. A. Cohn, J. F. Engelhardt, and J. M. Wilson. “An approach for treating the hepatobiliary disease of cystic fibrosis by somatic gene transfer.” Proc Natl Acad Sci U S A 90, no. 10 (May 15, 1993): 4601–5. https://doi.org/10.1073/pnas.90.10.4601.
    Full Text Link to Item
  - VAJANAPHANICH, M., U. KACHINTORN, K. E. BARRETT, J. A. COHN, K. DHARMSATHAPHORN, and A. TRAYNORKAPLAN. “PHOSPHATIDIC-ACID MODULATES CL(-) SECRETION IN T84-CELLS - VARYING EFFECTS DEPENDING ON MODE OF STIMULATION.” American Journal of Physiology 264, no. 5 (May 1, 1993): C1210–18.
    Link to Item
  - Vajanaphanich, M., U. Kachintorn, K. E. Barrett, J. A. Cohn, K. Dharmsathaphorn, and A. Traynor-Kaplan. “Phosphatidic acid modulates Cl- secretion in T84 cells: varying effects depending on mode of stimulation.” Am J Physiol 264, no. 5 Pt 1 (May 1993): C1210–18. https://doi.org/10.1152/ajpcell.1993.264.5.C1210.
    Full Text Link to Item
  - Strong, T. V., D. J. Wilkinson, M. K. Mansoura, D. C. Devor, K. Henze, Y. Yang, J. M. Wilson, J. A. Cohn, D. C. Dawson, and R. A. Frizzell. “Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance.” Hum Mol Genet 2, no. 3 (March 1993): 225–30. https://doi.org/10.1093/hmg/2.3.225.
    Full Text Link to Item
  - Basavappa, S., J. Middleton, A. W. Mangel, J. M. Mcgill, J. A. Cohn, and J. G. Fitz. “Cl^- and K⁺ transport in human biliary cell lines.” Gastroenterology 104, no. 6 (1993): 1796–1805.
  - Fitz, J. G., S. Basavappa, J. McGill, O. Melhus, and J. A. Cohn. “Regulation of membrane chloride currents in rat bile duct epithelial cells.” J Clin Invest 91, no. 1 (January 1993): 319–28. https://doi.org/10.1172/JCI116188.
    Full Text Link to Item
  - Engelhardt, J. F., J. R. Yankaskas, S. A. Ernst, Y. Yang, C. R. Marino, R. C. Boucher, J. A. Cohn, and J. M. Wilson. “Submucosal glands are the predominant site of CFTR expression in the human bronchus.” Nat Genet 2, no. 3 (November 1992): 240–48. https://doi.org/10.1038/ng1192-240.
    Full Text Link to Item
  - Whitsett, J. A., C. R. Dey, B. R. Stripp, K. A. Wikenheiser, J. C. Clark, S. E. Wert, R. J. Gregory, A. E. Smith, J. A. Cohn, and J. M. Wilson. “Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice.” Nat Genet 2, no. 1 (September 1992): 13–20. https://doi.org/10.1038/ng0992-13.
    Full Text Link to Item
  - Nguyen, T. D., G. G. Heintz, and J. A. Cohn. “Pituitary adenylate cyclase-activating polypeptide stimulates secretion in T84 cells.” Gastroenterology 103, no. 2 (August 1992): 539–44. https://doi.org/10.1016/0016-5085(92)90844-o.
    Full Text Link to Item
  - Picciotto, M. R., J. A. Cohn, G. Bertuzzi, P. Greengard, and A. C. Nairn. “Phosphorylation of the cystic fibrosis transmembrane conductance regulator.” J Biol Chem 267, no. 18 (June 25, 1992): 12742–52.
    Link to Item
  - Kwatra, M. M., D. D. Bigner, and J. A. Cohn. “The ligand binding domain of the epidermal growth factor receptor is not required for receptor dimerization.” Biochim Biophys Acta 1134, no. 2 (March 16, 1992): 178–81. https://doi.org/10.1016/0167-4889(92)90042-a.
    Full Text Link to Item
  - Cohn, J. A., A. C. Nairn, C. R. Marino, O. Melhus, and J. Kole. “Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line.” Proc Natl Acad Sci U S A 89, no. 6 (March 15, 1992): 2340–44. https://doi.org/10.1073/pnas.89.6.2340.
    Full Text Link to Item
  - Cohn, J. A., O. Melhus, L. J. Page, K. L. Dittrich, and S. R. Vigna. “CFTR: development of high- affinity antibodies and localization in sweat gland.” Biochem Biophys Res Commun 181, no. 1 (November 27, 1991): 36–43. https://doi.org/10.1016/s0006-291x(05)81378-6.
    Full Text Link to Item
  - Marino, C. R., L. M. Matovcik, F. S. Gorelick, and J. A. Cohn. “Localization of the cystic fibrosis transmembrane conductance regulator in pancreas.” J Clin Invest 88, no. 2 (August 1991): 712–16. https://doi.org/10.1172/JCI115358.
    Full Text Link to Item
  - Nguyen, T. D., A. T. Canada, G. G. Heintz, T. W. Gettys, and J. A. Cohn. “Stimulation of secretion by the T84 colonic epithelial cell line with dietary flavonols.” Biochem Pharmacol 41, no. 12 (June 15, 1991): 1879–86. https://doi.org/10.1016/0006-2952(91)90127-q.
    Full Text Link to Item
  - Schwartz, R. D., G. Heuschneider, P. P. Edgar, and J. A. Cohn. “cAMP analogs inhibit gamma-aminobutyric acid-gated chloride flux and activate protein kinase A in brain synaptoneurosomes.” Mol Pharmacol 39, no. 3 (March 1991): 370–75.
    Link to Item
  - Marino, C. R., L. M. Matovcik, F. S. Gorelick, and J. A. Cohn. “Erratum: (The Journal of Clinical Investigation, Volume 88, No. 2, August 1991).” Journal of Clinical Investigation 88, no. 4 (January 1, 1991): 1433.
  - BARRETT, K. E., J. A. COHN, P. A. HUOTT, S. I. WASSERMAN, and K. DHARMSATHAPHORN. “IMMUNE-RELATED INTESTINAL CHLORIDE SECRETION .2. EFFECT OF ADENOSINE ON T84 CELL-LINE.” American Journal of Physiology 258, no. 5 (May 1, 1990): C902–12.
    Link to Item
  - Barrett, K. E., J. A. Cohn, P. A. Huott, S. I. Wasserman, and K. Dharmsathaphorn. “Immune-related intestinal chloride secretion. II. Effect of adenosine on T84 cell line.” Am J Physiol 258, no. 5 Pt 1 (May 1990): C902–12. https://doi.org/10.1152/ajpcell.1990.258.5.C902.
    Full Text Link to Item
  - Levenson, R. M., G. M. Anderson, J. A. Cohn, and P. J. Blackshear. “Giant two-dimensional gel electrophoresis: methodological update and comparison with intermediate-format gel systems.” Electrophoresis 11, no. 3 (March 1990): 269–79. https://doi.org/10.1002/elps.1150110310.
    Full Text Link to Item
  - COHN, J. A. “PROTEIN KINASE-C MEDIATES CHOLINERGICALLY REGULATED PROTEIN-PHOSPHORYLATION IN A CL--SECRETING EPITHELIUM.” American Journal of Physiology 258, no. 2 (February 1, 1990): C227–33.
    Link to Item
  - Cohn, J. A. “Protein kinase C mediates cholinergically regulated protein phosphorylation in a Cl(-)-secreting epithelium.” Am J Physiol 258, no. 2 Pt 1 (February 1990): C227–33. https://doi.org/10.1152/ajpcell.1990.258.2.C227.
    Full Text Link to Item
  - Cohn, J. A., N. C. Dougherty, and W. F. King. “Histamine stimulates calcium-mediated protein phosphorylation in a colonic epithelial cell line.” Biochem Biophys Res Commun 165, no. 2 (December 15, 1989): 810–16. https://doi.org/10.1016/s0006-291x(89)80038-5.
    Full Text Link to Item
  - Dharmsathaphorn, K., J. Cohn, and G. Beuerlein. “Multiple calcium-mediated effector mechanisms regulate chloride secretory responses in T84-cells.” Am J Physiol 256, no. 6 Pt 1 (June 1989): C1224–30. https://doi.org/10.1152/ajpcell.1989.256.6.C1224.
    Full Text Link to Item
  - Cohn, J. A. “Vasoactive intestinal peptide stimulates protein phosphorylation in a colonic epithelial cell line.” American Journal of Physiology Gastrointestinal and Liver Physiology 253, no. 3 (December 1, 1987).
  - Cohn, J. A. “Vasoactive intestinal peptide stimulates protein phosphorylation in a colonic epithelial cell line.” Am J Physiol 253, no. 3 Pt 1 (September 1987): G420–24. https://doi.org/10.1152/ajpgi.1987.253.3.G420.
    Full Text Link to Item
  - Cohn, J. A., B. Kinder, J. D. Jamieson, N. G. Delahunt, and F. S. Gorelick. “Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas.” Biochim Biophys Acta 928, no. 3 (May 18, 1987): 320–31. https://doi.org/10.1016/0167-4889(87)90192-3.
    Full Text Link to Item
  - Cohn, J. A., F. S. Gorelick, N. G. Delahunt, and J. D. Jamieson. “Purification and characterization of a calmodulin-dependent kinase from rat pancreas.” Federation Proceedings 43, no. 6 (January 1, 1984).
  - Gorelick, F. S., J. A. Cohn, S. D. Freedman, N. G. Delahunt, J. M. Gershoni, and J. D. Jamieson. “Calmodulin-stimulated protein kinase activity from rat pancreas.” J Cell Biol 97, no. 4 (October 1983): 1294–98. https://doi.org/10.1083/jcb.97.4.1294.
    Full Text Link to Item
  - Cohn, J. A., and A. Cerami. “The influence of genetic background on the susceptibility of mice to diabetes induced by alloxan and on recovery from alloxan diabetes.” Diabetologia 17, no. 3 (September 1979): 187–91. https://doi.org/10.1007/BF01219747.
    Full Text Link to Item
  - Cohn, J. A., A. P. Alvares, and A. Kappas. “On the occurrence of cytochrome P-450 and aryl hydrocarbon hydroxylase activity in rat brain.” J Exp Med 145, no. 6 (June 1, 1977): 1607–11. https://doi.org/10.1084/jem.145.6.1607.
    Full Text Link to Item
- Conference Papers
  - Mansfield, S. G., R. Hawkins-Clark, J. A. Cohn, J. Kole, L. G. Mitchell, and M. A. Garcia-Blanco. “5 ' exon replacement by spliceosome mediated RNA trans-splicing.” In Molecular Therapy, 7:S301–2. ACADEMIC PRESS INC ELSEVIER SCIENCE, 2003.
    Link to Item
  - Feng, J., J. A. Cohn, J. P. Neoptolemus, Z. Jiang, W. Greenhalf, I. Ellis, and S. S. Sommer. “Comprehensive detection of CFTR mutations in idiopathic chronic pancreatitis.” In American Journal of Human Genetics, 71:483–483. UNIV CHICAGO PRESS, 2002.
    Link to Item
  - Mansfield, S. G., J. Kole, M. Puttaragu, C. C. Yang, M. A. Garcio-Blanco, L. G. Mitchell, and J. A. Cohn. “Repair of mutant CFTR mRNA by targeted RNA trans-splicing.” In Gastroenterology, 118:A185–A185. Elsevier BV, 2000. https://doi.org/10.1016/s0016-5085(00)82817-6.
    Full Text
  - Friedman, K. J., W. E. Highsmith, Z. Zhou, P. G. Noone, A. Spock, J. A. Cohn, L. M. Silverman, and M. R. Knowles. “D1152H: A common CFTR mutation associated with highly variable disease expression.” In American Journal of Human Genetics, 65:A295–A295. UNIV CHICAGO PRESS, 1999.
    Link to Item
  - Cohn, J. A., L. Chien, L. D. Howell, and J. Kole. “Heirarchical phosphorylation of CFTR serine-737.” In Gastroenterology, 116:A870–A870. W B SAUNDERS CO-ELSEVIER INC, 1999.
    Link to Item
  - Cohn, J. A., K. J. Friedman, L. M. Silverman, P. G. Noone, M. R. Knowles, and P. S. Jowell. “CFTR mutations predispose to chronic pancreatitis without cystic fibrosis lung disease.” In Gastroenterology, 112:A434–A434. W B SAUNDERS CO-ELSEVIER INC, 1997.
    Link to Item
  - Cohn, J. A., R. A. Borchardt, A. Y. R. Yu, and J. Kole. “Regulation of the nbd1/R domain of CFTR by ATP and by protein kinases.” In Gastroenterology, 112:A355–A355. W B SAUNDERS CO-ELSEVIER INC, 1997.
    Link to Item
  - COHN, J. A., T. D. NGUYEN, A. M. KAZ, and J. KOLE. “THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR IN RAT ENTEROCYTES.” In Gastroenterology, 106:A229–A229. W B SAUNDERS CO, 1994.
    Link to Item
  - BRADBURY, N. A., J. A. COHN, C. J. VENGLARIK, and R. J. BRIDGES. “THE CYSTIC-FIBROSIS GENE-PRODUCT (CFTR) IS IN ENDOCYTIC CLATHRIN COATED VESICLES.” In Faseb Journal, 7:A355–A355. FEDERATION AMER SOC EXP BIOL, 1993.
    Link to Item
  - FITZ, J. G., J. MCGILL, S. BASAVAPPA, and J. A. COHN. “BILE-DUCT EPITHELIAL-CELLS CONTAIN REGULATED CHLORIDE CHANNELS AND CFTR.” In Clinical Research, 40:A319–A319. SLACK INC, 1992.
    Link to Item
  - COHN, J. A. “CAMP-DEPENDENT C1- TRANSPORT REQUIRES THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR IN T84 CELLS.” In Clinical Research, 39:A301–A301. SLACK INC, 1991.
    Link to Item
  - INGBAR, D. H., J. A. COHN, E. JACOBSON, and C. R. MARINO. “DEVELOPMENTAL REGULATION OF CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR CONTENT IN RAT LUNG.” In Clinical Research, 39:A149–A149. SLACK INC, 1991.
    Link to Item
  - COHN, J. A. “REGULATION OF CYCLIC AMP-MEDIATED AND CALCIUM-MEDIATED PROTEIN-PHOSPHORYLATION BY BILE-ACIDS IN T84 CELL MONOLAYERS.” In Clinical Research, 38:A444–A444. SLACK INC, 1990.
    Link to Item

Some information on this profile has been compiled automatically from Duke databases and external sources. (Our About page explains how this works.) If you see a problem with the information, please write to Scholars@Duke and let us know. We will reply promptly.

Education, Training, & Certifications

Previous Appointments & Affiliations

Selected Grants

Selected Publications

Academic Articles

Conference Papers