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Jonathan Allen Cohn

Jonathan Allen Cohn

Professor of Medicine
Medicine, Gastroenterology
jonathan.cohn@duke.edu
DUMC Box 3256, Durham, NC 27710
GSRB-1 Room 2038, 905 S LaSalle St, Durham, NC 27710

Selected Publications

Hereditary Diseases of the Pancreas

Journal Article · February 17, 2009 Full text Cite

Pancreatic exocrine dysfunction

Journal Article · December 1, 2006 Pancreatic exocrine dysfunction can present clinically either as an acute illness, a destructive process, or a condition such as pancreatic insufficiency or chronic abdominal pain. Acute pancreatitis is associated with injury to the pancreas through direct ... Full text Cite

Increased risk of idiopathic chronic pancreatitis in cystic fibrosis carriers.

Journal Article Hum Mutat · October 2005 Cystic fibrosis (CF) is a recessive disease caused by mutations of the CF transmembrane conductance regulator (CFTR) gene. The risk of idiopathic chronic pancreatitis (ICP) is increased in individuals who have CFTR genotypes containing a CF-causing mutatio ... Full text Link to item Cite

A word of thanks.

Journal Article Gastroenterology · September 2005 Full text Link to item Cite

Reduced CFTR function and the pathobiology of idiopathic pancreatitis.

Journal Article J Clin Gastroenterol · April 2005 Idiopathic chronic pancreatitis (ICP) is the leading cause of chronic pancreatitis in children and nonalcoholic adults. The risk of developing ICP is increased in individuals who have mutations of the cystic fibrosis gene (CFTR) and of a trypsin inhibitor ... Full text Link to item Cite

The impact of cystic fibrosis and PSTI/SPINK1 gene mutations on susceptibility to chronic pancreatitis.

Journal Article Clin Lab Med · March 2005 This article reviews current concepts regarding the pathobiology of cystic fibrosis pancreatic disease. It summarizes recent studies on the relationship between CFTR mutations and pancreatitis, and it reviews several unresolved issues in the field. ... Full text Link to item Cite

The role of cystic fibrosis gene mutations in determining susceptibility to chronic pancreatitis.

Journal Article Gastroenterol Clin North Am · December 2004 This article reviews current concepts regarding the pathobiology of cystic fibrosis pancreatic disease. It summarizes recent studies on the relationship between CFTR mutations and pancreatitis, and it reviews several unresolved issues in the field. ... Full text Link to item Cite

Protein kinase A regulates ATP hydrolysis and dimerization by a CFTR (cystic fibrosis transmembrane conductance regulator) domain.

Journal Article Biochem J · February 15, 2004 Gating of the CFTR Cl- channel is associated with ATP hydrolysis at the nucleotide-binding domains (NBD1, NBD2) and requires PKA (protein kinase A) phosphorylation of the R domain. The manner in which the NBD1, NBD2 and R domains of CFTR (cystic fibrosis t ... Full text Link to item Cite

5' exon replacement and repair by spliceosome-mediated RNA trans-splicing.

Journal Article RNA · October 2003 Spliceosome-mediated RNA trans-splicing (SMaRT) has been used previously to reprogram mutant endogenous CFTR and factor VIII mRNAs in human epithelial cell and tissue models and knockout mice, respectively. Those studies used 3' exon replacement (3'ER); a ... Full text Link to item Cite

Agonist-induced coordinated trafficking of functionally related transport proteins for water and ions in cholangiocytes.

Journal Article J Biol Chem · May 30, 2003 We previously proposed that ductal bile formation is regulated by secretin-responsive relocation of aquaporin 1 (AQP1), a water-selective channel protein, from an intracellular vesicular compartment to the apical membrane of cholangiocytes. In this study, ... Full text Link to item Cite

Lessons from Mendel about data analysis.

Journal Article Gastroenterology · April 2003 Full text Link to item Cite

Motion--genetic testing is useful in the diagnosis of nonhereditary pancreatic conditions: arguments against the motion.

Journal Article Can J Gastroenterol · January 2003 Mutations of two genes, the cystic fibrosis transmembrane conductance regulator gene (CFTR) and the pancreatic secretory trypsin inhibitor gene (PSTI), are associated with an increase in the risk of idiopathic chronic pancreatitis. Persons who have mutatio ... Full text Link to item Cite

Comprehensive detection of CFTR mutations in idiopathic chronic pancreatitis.

Conference AMERICAN JOURNAL OF HUMAN GENETICS · October 1, 2002 Link to item Cite

Idiopathic pancreatitis related to CFTR: complex inheritance and identification of a modifier gene.

Journal Article J Investig Med · September 2002 Idiopathic chronic pancreatitis (ICP) is the leading cause of nonalcoholic chronic pancreatitis. This study examined a series of patients with ICP to determine the prevalence and role of mutations of the cystic fibrosis gene (CFTR) and of a trypsin inhibit ... Full text Link to item Cite

Partial correction of endogenous DeltaF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing.

Journal Article Nat Biotechnol · January 2002 Spliceosome-mediated RNA trans-splicing (SMaRT) was investigated as a means for functionally correcting endogenous DeltaF508 cystic fibrosis transmembrane conductance regulator (CFTR) transcripts using in vitro human cystic fibrosis (CF) polarized airway e ... Full text Link to item Cite

Cystic fibrosis gene mutations and pancreatitis risk: relation to epithelial ion transport and trypsin inhibitor gene mutations.

Journal Article Gastroenterology · December 2001 Featured Publication BACKGROUND & AIMS: Nonalcoholic chronic pancreatitis is usually idiopathic and often associated with cystic fibrosis gene (CFTR) mutations. It is unknown whether pancreatitis risk correlates with having 1 or 2 CFTR mutations, abnormal epithelial ion transp ... Full text Link to item Cite

Activation of G551D CFTR channel with MPB-91: regulation by ATPase activity and phosphorylation.

Journal Article Am J Physiol Cell Physiol · November 2001 We have designed and synthesized benzo[c]quinolizinium derivatives and evaluated their effects on the activity of G551D cystic fibrosis transmembrane conductance regulator (CFTR) expressed in Chinese hamster ovary and Fisher rat thyroid cells. We demonstra ... Full text Link to item Cite

Cystic fibrosis transmembrane regulator regulates uptake of sphingoid base phosphates and lysophosphatidic acid: modulation of cellular activity of sphingosine 1-phosphate.

Journal Article J Biol Chem · September 21, 2001 Sphingolipids have been implicated in the regulation of cell growth, differentiation, and programmed cell death. Sphingosine 1-phosphate (SPP) has recently emerged as an important lipid messenger and a ligand for the endothelial differentiation gene recept ... Full text Link to item Cite

ENaC- and CFTR-dependent ion and fluid transport in mammary epithelia.

Journal Article Am J Physiol Cell Physiol · August 2001 Mammary epithelial 31EG4 cells (MEC) were grown as monolayers on filters to analyze the apical membrane mechanisms that help mediate ion and fluid transport across the epithelium. RT-PCR showed the presence of cystic fibrosis transmembrane conductance regu ... Full text Link to item Cite

The diagnosis of CF in patients with non-classical phenotypes

Journal Article Pediatric Pulmonology · December 1, 2000 Cite

Repair of CFTR mRNA by spliceosome-mediated RNA trans-splicing.

Journal Article Gene Ther · November 2000 Most messenger RNA precursors (pre-mRNA) undergo cis-splicing in which introns are excised and the adjoining exons from a single pre-mRNA are ligated together to form mature messenger RNA. This reaction is driven by a complex known as the spliceosome. Spli ... Full text Link to item Cite

ATP hydrolysis by a CFTR domain: pharmacology and effects of G551D mutation.

Journal Article Biochem Biophys Res Commun · May 10, 2000 Residues 417-830 of the cystic fibrosis transmembrane conductance regulator (CFTR) were expressed as a glutathione-S-transferase fusion protein. This fusion protein, NBD1/R/GST, contains the regulatory and first nucleotide binding domains of CFTR. NBD1/R/G ... Full text Link to item Cite

Cystic fibrosis mutations and genetic predisposition to idiopathic chronic pancreatitis.

Journal Article Med Clin North Am · May 2000 Idiopathic chronic pancreatitis is a leading cause of chronic pancreatitis. Work from this and other groups has shown that idiopathic chronic pancreatitis is associated with mutations of the cystic fibrosis gene (CFTR). Many idiopathic pancreatitis patient ... Full text Link to item Cite

Inherited diseases of the pancreas: Preface

Journal Article Medical Clinics of North America · January 1, 2000 Full text Cite

Correction of aberrant splicing of the cystic fibrosis transmembrane conductance regulator (CFTR) gene by antisense oligonucleotides.

Journal Article J Biol Chem · December 17, 1999 The CFTR splicing mutation 3849 + 10 kb C --> T creates a novel donor site 10 kilobases (kb) into intron 19 of the gene and is one of the more common splicing mutations that causes cystic fibrosis (CF). It has an elevated prevalence among patients with aty ... Full text Link to item Cite

D1152H: A common CFTR mutation associated with highly variable disease expression.

Conference AMERICAN JOURNAL OF HUMAN GENETICS · October 1, 1999 Link to item Cite

CFTR mutations and idiopathic chronic pancreatitis

Journal Article Pediatric Pulmonology · September 1, 1999 Cite

Is idiopathic chronic pancreatitis a form of cystic fibrosis?

Journal Article Pediatric Pulmonology · September 1, 1999 Cite

Are mutations in the cystic fibrosis gene important in chronic pancreatitis?

Journal Article Surg Clin North Am · August 1999 The leading causes of chronic pancreatitis are alcohol and idiopathic pancreatitis. The importance of genetic factors in chronic pancreatitis has been uncertain. Recently, however, it was learned that many patients with idiopathic chronic pancreatitis have ... Full text Link to item Cite

Cystic fibrosis in the pancreas: recent advances provide new insights.

Journal Article Curr Gastroenterol Rep · April 1999 Idiopathic chronic pancreatitis accounts for up to one third of chronic pancreatitis cases. The most common inherited disease of the exocrine pancreas is cystic fibrosis, which is caused by mutations of a gene encoding an ion transport protein. It was disc ... Full text Link to item Cite

Heirarchical phosphorylation of CFTR serine-737

Conference GASTROENTEROLOGY · April 1, 1999 Link to item Cite

Mutations of the cystic fibrosis gene and pancreatitis - Reply

Journal Article NEW ENGLAND JOURNAL OF MEDICINE · January 21, 1999 Link to item Cite

Relation between mutations of the cystic fibrosis gene and idiopathic pancreatitis.

Journal Article N Engl J Med · September 3, 1998 Featured Publication BACKGROUND: It is unknown whether genetic factors predispose patients to idiopathic pancreatitis. In patients with cystic fibrosis, mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene typically cause pulmonary and pancreatic in ... Full text Link to item Cite

Structural cues involved in endoplasmic reticulum degradation of G85E and G91R mutant cystic fibrosis transmembrane conductance regulator.

Journal Article J Clin Invest · September 1, 1997 Abnormal folding of mutant cystic fibrosis transmembrane conductance regulator (CFTR) and subsequent degradation in the endoplasmic reticulum is the basis for most cases of cystic fibrosis. Structural differences between wild-type (WT) and mutant proteins, ... Full text Link to item Cite

Protein kinase C and Ca2+ activation of mucin secretion in airway goblet cells.

Journal Article Am J Physiol · July 1997 Airway goblet cells secrete mucin in response to ATP and uridine 5'-triphosphate (UTP), but the underlying signal transduction pathways are poorly understood. Cultures of SPOC1 cells (L. H. Abdullah, S. W. Davis, L. Burch, M. Yamauchi, S. H. Randell, P. Ne ... Full text Link to item Cite

Protein kinase C and Ca2+ activation of mucin secretion in airway goblet cells

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY · July 1, 1997 Link to item Cite

Gene targeting of a CFTR allele in HT29 human epithelial cells.

Journal Article J Cell Physiol · March 1997 HT29 cells endogenously express the cystic fibrosis transmembrane conductance regulator (CFTR) and have been used previously as a model to examine cellular regulation of CFTR expression and chloride secretory function. Homologous recombination has been use ... Full text Link to item Cite

Polarized apical-membrane expression of cAMP-actlvated chloride channels in isolated epithelial cells

Journal Article FASEB Journal · December 1, 1996 We tested the hypothesis that cAMP-stimulated CI channels are expressed exclusively on the apical membrane of isolated polarized cells (figure-eight cells) from Necturus gallbladder (NGB) epithelium. In the intact NGB epithelium, the electrophysiological c ... Cite

Polarized expression of cAMP-activated chloride channels in isolated epithelial cells.

Journal Article Am J Physiol · November 1996 We have described a preparation of Necturus maculosus gallbladder (NGB) epithelium yielding isolated cells that retain structural and functional polarity ("figure-eight" cells). These cells have a normal membrane voltage and remain polarized for several ho ... Full text Link to item Cite

Polarized expression of cAMP-activated chloride channels in isolated epithelial cells

Journal Article AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY · November 1, 1996 Link to item Cite

CFTR as a cAMP-dependent regulator of sodium channels.

Journal Article Science · August 11, 1995 Featured Publication Cystic fibrosis transmembrane regulator (CFTR), the gene product that is mutated in cystic fibrosis (CF) patients, has a well-recognized function as a cyclic adenosine 3',5'-monophosphate (cAMP)-regulated chloride channel, but this property does not accoun ... Full text Link to item Cite

Dephosphorylation of ezrin as an early event in renal microvillar breakdown and anoxic injury.

Journal Article Proc Natl Acad Sci U S A · August 1, 1995 Disruption of the renal proximal tubule (PT) brush border is a prominent early event during ischemic injury to the kidney. The molecular basis for this event is unknown. Within the brush border, ezrin may normally link the cytoskeleton to the cell plasma m ... Full text Link to item Cite

Missense mutation (G480C) in the CFTR gene associated with protein mislocalization but normal chloride channel activity.

Journal Article Hum Mol Genet · February 1995 We have identified a novel CFTR missense mutation associated with a protein trafficking defect in mammalian cells but normal chloride channel properties in a Xenopus oocyte assay. The mutation, a cysteine for glycine substitution at residue 480 (G480C), wa ... Full text Link to item Cite

Salt stress increases abundance and glycosylation of CFTR localized at apical surfaces of salt gland secretory cells.

Journal Article Am J Physiol · October 1994 Osmotic stress elicits hypertonic NaCl secretion and promotes structural and biochemical differentiation in avian salt glands. In addition to cholinergic control, Cl- secretion is stimulated by vasoactive intestinal peptide (VIP), suggesting that the cysti ... Full text Link to item Cite

Biochemical and biophysical identification of cystic fibrosis transmembrane conductance regulator chloride channels as components of endocytic clathrin-coated vesicles.

Journal Article J Biol Chem · March 18, 1994 Cystic fibrosis results from mutations in the gene encoding the CFTR Cl- channel. Although CFTR occurs as an integral component of the plasma membrane, recent studies implicate CFTR in endocytic recycling and suggest that the protein may also exist in intr ... Link to item Cite

Expression of the cystic fibrosis gene in adult human lung.

Journal Article J Clin Invest · February 1994 Critical to an understanding of the pulmonary disease in cystic fibrosis (CF) and the development of effective gene therapies is a definition of the distribution and regulation of CF gene expression in adult human lung. Previous studies have detected the p ... Full text Link to item Cite

Salt stress increases abundance and glycosylation of CFTR localized at apical surfaces of salt gland secretory cells

Journal Article American Journal of Physiology - Cell Physiology · January 1, 1994 Osmotic stress elicits hypertonic NaCl secretion and promotes structural and biochemical differentiation in avian salt glands. In addition to cholinergic control, Cl- secretion is stimulated by vasoactive intestinal peptide (VIP), suggesting that the cysti ... Cite

Localization of the cystic fibrosis transmembrane conductance regulator in human bile duct epithelial cells.

Journal Article Gastroenterology · December 1993 Featured Publication BACKGROUND: Liver dysfunction is a common manifestation of cystic fibrosis (CF), a disease caused by mutations affecting the CF transmembrane conductance regulator (CFTR). The aim of this study was to examine the distribution and role of CFTR in liver. MET ... Full text Link to item Cite

The common variant of cystic fibrosis transmembrane conductance regulator is recognized by hsp70 and degraded in a pre-Golgi nonlysosomal compartment.

Journal Article Proc Natl Acad Sci U S A · October 15, 1993 The most common cause of cystic fibrosis is deletion of Phe-508 (delta F508) from the cystic fibrosis transmembrane conductance regulator (CFTR). Previous studies have suggested that delta F508 CFTR is an unstable protein that retains a pattern of glycosyl ... Full text Link to item Cite

Protein phosphorylation responses in normal and cystic fibrosis airway epithelial cell lines.

Journal Article Am J Respir Cell Mol Biol · October 1993 Monolayers of SV-40 immortalized human airway epithelial cell lines were stimulated with bradykinin and isoproterenol to study protein phosphorylation responses that accompany ion transport regulation in normal (BEAS) and cystic fibrosis (CF/T43) cells. Ph ... Full text Link to item Cite

Molecular basis of defective anion transport in L cells expressing recombinant forms of CFTR.

Journal Article Hum Mol Genet · August 1993 Cystic fibrosis (CF) is caused by mutations in the gene encoding a chloride channel called the CF transmembrane conductance regulator (CFTR). A single mutation in this gene, deletion of three nucleotides that leads to the absence of phenylalanine 508 (i.e. ... Full text Link to item Cite

Cl- and K+ transport in human biliary cell lines.

Journal Article Gastroenterology · June 1993 BACKGROUND: The cellular mechanisms which contribute to billing secretion and absorption are not fully defined. The purpose of these studies was to evaluate the membrane ion transport properties of Mz-ChA-1 and Sk-ChA-1 cell lines derived from human biliar ... Full text Link to item Cite

An approach for treating the hepatobiliary disease of cystic fibrosis by somatic gene transfer.

Journal Article Proc Natl Acad Sci U S A · May 15, 1993 Cystic fibrosis (CF) is an inherited disease of epithelial cell ion transport that is associated with pathology in multiple organ systems, including lung, pancreas, and liver. As treatment of the pulmonary manifestations of CF has improved, management of C ... Full text Link to item Cite

Phosphatidic acid modulates Cl- secretion in T84 cells: varying effects depending on mode of stimulation.

Journal Article Am J Physiol · May 1993 Cl- secretion in T84 cells evoked by a stimulus that activates protein kinase C, carbachol, was associated with elevated levels of 32P-labeled phosphatidic acid (PA). PA's role in the regulation of Cl- secretion was explored by examining the effect of exog ... Full text Link to item Cite

Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance.

Journal Article Hum Mol Genet · March 1993 The cystic fibrosis transmembrane conductance regulator (CFTR) gene encodes a cAMP-activated chloride (Cl-) channel, and expression of the full length gene in vitro is sufficient to correct the Cl- conductance defect that is characteristic of cystic fibros ... Full text Link to item Cite

Regulation of membrane chloride currents in rat bile duct epithelial cells.

Journal Article J Clin Invest · January 1993 Featured Publication This study examines the conductive properties of the plasma membrane of cells isolated from the intrahepatic portion of bile ducts. Membrane Cl- conductance was measured in single cells using whole-cell patch clamp recording techniques and in cells in shor ... Full text Link to item Cite

Cl- and K+ transport in human biliary cell lines

Journal Article Gastroenterology · 1993 Background: The cellular mechanisms which contribute to billing secretion and absorption are not fully defined. The purpose of these studies was to evaluate the membrane ion transport properties of Mz-ChA-1 and Sk-ChA-1 cell lines derived from human biliar ... Cite

Submucosal glands are the predominant site of CFTR expression in the human bronchus.

Journal Article Nat Genet · November 1992 We have used in situ hybridization and immunocytochemistry to characterize the cellular distribution of cystic fibrosis (CF) gene expression in human bronchus. The cystic fibrosis transmembrane conductance regular (CFTR) was primarily localized to cells of ... Full text Link to item Cite

Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice.

Journal Article Nat Genet · September 1992 Human cystic fibrosis transmembrane conductance regulator (CFTR) was expressed in transgenic mice under the control of transcriptional elements derived from the human surfactant protein C (SP-C) gene. The hCFTR mRNA was expressed in lungs and testes: in th ... Full text Link to item Cite

Pituitary adenylate cyclase-activating polypeptide stimulates secretion in T84 cells.

Journal Article Gastroenterology · August 1992 Pituitary adenylate-cyclase-activating peptide (PA-CAP) and PACAP-27 are novel hypothalamic peptides that can stimulate adenylate cyclase in cultured anterior pituitary cells. Because these peptides are present in the gut and are homologous with vasoactive ... Full text Link to item Cite

Phosphorylation of the cystic fibrosis transmembrane conductance regulator.

Journal Article J Biol Chem · June 25, 1992 Regulation of epithelial chloride flux, which is defective in patients with cystic fibrosis, may be mediated by phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR) by cyclic AMP-dependent protein kinase (PKA) or protein kinase ... Link to item Cite

The ligand binding domain of the epidermal growth factor receptor is not required for receptor dimerization.

Journal Article Biochim Biophys Acta · March 16, 1992 To examine the role of the ligand binding domain of epidermal growth factor receptor in its dimerization, we studied the dimerization of a truncated form of the receptor that resembles v-erbB in that it lacks a ligand binding domain. Receptor dimerization ... Full text Link to item Cite

Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line.

Journal Article Proc Natl Acad Sci U S A · March 15, 1992 Featured Publication An anti-peptide antibody raised to the C-terminal sequence of the cystic fibrosis transmembrane conductance regulator (CFTR) was used to examine CFTR immunoreactivity in the T84 colonocyte cell line. Immunoblots of T84 cell lysates detected CFTR as a 170-k ... Full text Link to item Cite

CFTR: development of high- affinity antibodies and localization in sweat gland.

Journal Article Biochem Biophys Res Commun · November 27, 1991 Rabbit antisera were raised to six synthetic peptides corresponding to amino acid sequences contained in the protein product of the cystic fibrosis gene, CFTR. For two peptides, [Lys102]CFTR(102-116) and CFTR(1468-1480), antibody-peptide binding was of hig ... Full text Link to item Cite

Localization of the cystic fibrosis transmembrane conductance regulator in pancreas.

Journal Article J Clin Invest · August 1991 Featured Publication Cystic fibrosis (CF) is characterized by an abnormality in cAMP-regulated chloride transport that results from a primary defect in the protein product of the CF gene, the CF transmembrane conductance regulator (CFTR). In this report, antibodies against CFT ... Full text Link to item Cite

Stimulation of secretion by the T84 colonic epithelial cell line with dietary flavonols.

Journal Article Biochem Pharmacol · June 15, 1991 Flavonols are dietary compounds widely distributed in plants and characterized by a 2-phenyl-benzo(alpha)pyrane nucleus possessing hydroxyl and ketone groups at positions 3 and 4, respectively. Kaempferol, quercetin, and myricetin are flavonols that are fu ... Full text Link to item Cite

cAMP analogs inhibit gamma-aminobutyric acid-gated chloride flux and activate protein kinase A in brain synaptoneurosomes.

Journal Article Mol Pharmacol · March 1991 The effects of permeant cAMP analogs were studied on the function of the gamma-aminobutyric acidA (GABAA) receptor and on the activation of protein kinase A in brain synaptoneurosomes. Incubation of cerebral cortical synaptoneurosomes with permeant cAMP an ... Link to item Cite

Erratum: (The Journal of Clinical Investigation, Volume 88, No. 2, August 1991)

Journal Article Journal of Clinical Investigation · January 1, 1991 Cite

Immune-related intestinal chloride secretion. II. Effect of adenosine on T84 cell line.

Journal Article Am J Physiol · May 1990 The inflammatory mediator adenosine caused sustained Cl- secretion across monolayers of T84 cells. The effect was promptly reversed by the adenosine receptor antagonist 8-phenyltheophylline and appeared to be mediated through an adenosine A2-receptor [rank ... Full text Link to item Cite

Giant two-dimensional gel electrophoresis: methodological update and comparison with intermediate-format gel systems.

Journal Article Electrophoresis · March 1990 Two-dimensional (2-D) gel electrophoresis methods for separating complex mixtures of proteins have not changed fundamentally since their original description in the late 1970's. Nevertheless, 2-D gel resolution has improved substantially as a result of a s ... Full text Link to item Cite

Protein kinase C mediates cholinergically regulated protein phosphorylation in a Cl(-)-secreting epithelium.

Journal Article Am J Physiol · February 1990 T84 cell monolayers were used to study the cholinergic regulation of protein phosphorylation in epithelial cells. When T84 cell monolayers are labeled with 32Pi and stimulated with carbachol, six proteins exhibit altered phosphorylation. The most prominent ... Full text Link to item Cite

Histamine stimulates calcium-mediated protein phosphorylation in a colonic epithelial cell line.

Journal Article Biochem Biophys Res Commun · December 15, 1989 Protein phosphorylation responses in intact enterocytes were examined by stimulating 32Pi-labeled T84 cell monolayers with histamine and resolving proteins by two-dimensional gel electrophoresis. Histamine increases 32P-incorporation into two acidic protei ... Full text Link to item Cite

Multiple calcium-mediated effector mechanisms regulate chloride secretory responses in T84-cells.

Journal Article Am J Physiol · June 1989 Free cytosolic Ca2+ [( Ca2+]i) has been implicated as a second messenger mediating the ion transport effects of carbachol, histamine, taurodeoxycholate, ionomycin, and 4-bromo-A23187 (4-BrA23187) in T84-cells. In this study, we correlated short-circuit cur ... Full text Link to item Cite

Vasoactive intestinal peptide stimulates protein phosphorylation in a colonic epithelial cell line

Journal Article American Journal of Physiology Gastrointestinal and Liver Physiology · December 1, 1987 The T84 colonic epithelial cell line was used to examine protein phosphorylation during neurohumoral stimulation of ion transport. T84 cell monolayers grown on collagen-coated filters were mounted in Ussing chambers to measure ion tra ... Cite

Vasoactive intestinal peptide stimulates protein phosphorylation in a colonic epithelial cell line.

Journal Article Am J Physiol · September 1987 The T84 colonic epithelial cell line was used to examine protein phosphorylation during neurohumoral stimulation of ion transport. T84 cell monolayers grown on collagen-coated filters were mounted in Ussing chambers to measure ion transport stimulated by v ... Full text Link to item Cite

Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas.

Journal Article Biochim Biophys Acta · May 18, 1987 A calcium/calmodulin-dependent protein kinase (Ca/calmodulin protein kinase) was purified from rat pancreas using hydrophobic chromatography followed by gel filtration and affinity chromatography. Ca/calmodulin protein kinase from pancreas resembled previo ... Full text Link to item Cite

Purification and characterization of a calmodulin-dependent kinase from rat pancreas

Journal Article Federation Proceedings · January 1, 1984 Cite

Calmodulin-stimulated protein kinase activity from rat pancreas.

Journal Article J Cell Biol · October 1983 Previous work from our laboratory has demonstrated that neurohumoral stimulation of the exocrine pancreas is associated with the phosphorylation of the Mr 29,000 ribosomal protein S6. In a cell-free system using pancreatic postmicrosomal supernatant as the ... Full text Link to item Cite

On the occurrence of cytochrome P-450 and aryl hydrocarbon hydroxylase activity in rat brain.

Journal Article J Exp Med · June 1, 1977 The difference spectra of the carbon monoxide-complex of dithionite-reduced rat brain microsomes, compared with both reduced microsomes, alone, and the carbon monoxide-complex of oxidized microsomes, indicate the presence of small amounts of cytochrome P-4 ... Full text Link to item Cite