Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance.

Publication ,  Journal Article
Strong, TV; Wilkinson, DJ; Mansoura, MK; Devor, DC; Henze, K; Yang, Y; Wilson, JM; Cohn, JA; Dawson, DC; Frizzell, RA
Published in: Hum Mol Genet
March 1993

The cystic fibrosis transmembrane conductance regulator (CFTR) gene encodes a cAMP-activated chloride (Cl-) channel, and expression of the full length gene in vitro is sufficient to correct the Cl- conductance defect that is characteristic of cystic fibrosis (CF) epithelial cells. Alternatively spliced forms of CFTR mRNA have been identified in several tissues from normal individuals. One of the alternative transcripts, often present at high levels, results in the in-frame deletion of exon 9. Translation of this transcript would result in a CFTR protein missing the amino terminal portion of the first nucleotide binding fold (NBF). To evaluate the possible function of this form of CFTR, a cDNA representing this transcript (CFTR delta 9) was transduced into CFPAC cells, which are derived from a CF patient. CFTR delta 9 RNA was expressed in the transduced cell lines, but only immature, incompletely glycosylated protein was detectable by Western blot analysis. No increase in cAMP-activated anion permeability was detectable by 125I efflux assay or by means of the halide sensitive dye 6-methoxy-N-(3-sulfopropyl) quinolinium (SPQ). In a second assay system, in vitro synthesized mRNA representing CFTR delta D9 was injected into Xenopus oocytes, but expression of this alternatively spliced form of CFTR was not associated with the appearance of Cl- conductance. These results suggest that the protein produced by the CFTR delta 9 transcript is not properly processed and is not capable of generating Cl- conductance in response to cAMP. Whether this alternative transcript has some other function or represents 'noise' in the mRNA splicing mechanism remains unresolved.

Duke Scholars

Published In

Hum Mol Genet

DOI

ISSN

0964-6906

Publication Date

March 1993

Volume

2

Issue

3

Start / End Page

225 / 230

Location

England

Related Subject Headings

  • Xenopus
  • RNA, Messenger
  • Oocytes
  • Molecular Sequence Data
  • Membrane Proteins
  • In Vitro Techniques
  • Humans
  • Genetics & Heredity
  • Female
  • Electric Conductivity
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Strong, T. V., Wilkinson, D. J., Mansoura, M. K., Devor, D. C., Henze, K., Yang, Y., … Frizzell, R. A. (1993). Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance. Hum Mol Genet, 2(3), 225–230. https://doi.org/10.1093/hmg/2.3.225
Strong, T. V., D. J. Wilkinson, M. K. Mansoura, D. C. Devor, K. Henze, Y. Yang, J. M. Wilson, J. A. Cohn, D. C. Dawson, and R. A. Frizzell. “Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance.Hum Mol Genet 2, no. 3 (March 1993): 225–30. https://doi.org/10.1093/hmg/2.3.225.
Strong TV, Wilkinson DJ, Mansoura MK, Devor DC, Henze K, Yang Y, Wilson JM, Cohn JA, Dawson DC, Frizzell RA. Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance. Hum Mol Genet. 1993 Mar;2(3):225–230.
Journal cover image

Published In

Hum Mol Genet

DOI

ISSN

0964-6906

Publication Date

March 1993

Volume

2

Issue

3

Start / End Page

225 / 230

Location

England

Related Subject Headings

  • Xenopus
  • RNA, Messenger
  • Oocytes
  • Molecular Sequence Data
  • Membrane Proteins
  • In Vitro Techniques
  • Humans
  • Genetics & Heredity
  • Female
  • Electric Conductivity