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Mediator-free amperometric determination of toxic substances based on their inhibition of immobilized horseradish peroxidase.

Publication ,  Journal Article
Zhao, J; Henkens, RW; Crumbliss, AL
Published in: Biotechnology progress
September 1996

We have demonstrated the feasibility of quantitatively detecting selected toxic materials through their inhibitory effect on an enzyme electrode that utilizes colloidal gold-immobilized horseradish peroxidase and does not require a mediator. Quantitative detection of azide, cyanide, thiourea, sulfide, and dichromate is demonstrated. The sensitivity and inhibition kinetics for this immobilized enzyme electrode are found to be different from those observed previously for homogeneous horseradish peroxidase. Possible reasons for this difference are discussed. Due to the availability of a large number of enzymes and their toxic inhibitors, this work based on immobilized enzyme inhibition coupled to an electrode surface significantly broadens the possible applications of biosensors and offers alternative methods for toxic substance determination.

Duke Scholars

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Published In

Biotechnology progress

DOI

EISSN

1520-6033

ISSN

8756-7938

Publication Date

September 1996

Volume

12

Issue

5

Start / End Page

703 / 708

Related Subject Headings

  • Toxicology
  • Kinetics
  • Horseradish Peroxidase
  • Hazardous Substances
  • Enzymes, Immobilized
  • Electrodes
  • Electrochemistry
  • Cations
  • Biotechnology
  • Anions
 

Citation

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Zhao, J., Henkens, R. W., & Crumbliss, A. L. (1996). Mediator-free amperometric determination of toxic substances based on their inhibition of immobilized horseradish peroxidase. Biotechnology Progress, 12(5), 703–708. https://doi.org/10.1021/bp960056q
Zhao, J., R. W. Henkens, and A. L. Crumbliss. “Mediator-free amperometric determination of toxic substances based on their inhibition of immobilized horseradish peroxidase.Biotechnology Progress 12, no. 5 (September 1996): 703–8. https://doi.org/10.1021/bp960056q.
Zhao, J., et al. “Mediator-free amperometric determination of toxic substances based on their inhibition of immobilized horseradish peroxidase.Biotechnology Progress, vol. 12, no. 5, Sept. 1996, pp. 703–08. Epmc, doi:10.1021/bp960056q.
Zhao J, Henkens RW, Crumbliss AL. Mediator-free amperometric determination of toxic substances based on their inhibition of immobilized horseradish peroxidase. Biotechnology progress. 1996 Sep;12(5):703–708.
Journal cover image

Published In

Biotechnology progress

DOI

EISSN

1520-6033

ISSN

8756-7938

Publication Date

September 1996

Volume

12

Issue

5

Start / End Page

703 / 708

Related Subject Headings

  • Toxicology
  • Kinetics
  • Horseradish Peroxidase
  • Hazardous Substances
  • Enzymes, Immobilized
  • Electrodes
  • Electrochemistry
  • Cations
  • Biotechnology
  • Anions