Morphogenesis in skin is governed by discrete sets of differentially expressed microRNAs.

Published

Journal Article

During embryogenesis, multipotent progenitors within the single-layered surface epithelium differentiate to form the epidermis and its appendages. Here, we show that microRNAs (miRNAs) have an essential role in orchestrating these events. We cloned more than 100 miRNAs from skin and show that epidermis and hair follicles differentially express discrete miRNA families. To explore the functional significance of this finding, we conditionally targeted Dicer1 gene ablation in embryonic skin progenitors. Within the first week after loss of miRNA expression, cell fate specification and differentiation were not markedly impaired, and in the interfollicular epidermis, apoptosis was not markedly increased. Notably, however, developing hair germs evaginate rather than invaginate, thereby perturbing the epidermal organization. Here we characterize miRNAs in skin, the existence of which was hitherto unappreciated, and demonstrate their differential expression and importance in the morphogenesis of epithelial tissues within this vital organ.

Full Text

Duke Authors

Cited Authors

  • Yi, R; O'Carroll, D; Pasolli, HA; Zhang, Z; Dietrich, FS; Tarakhovsky, A; Fuchs, E

Published Date

  • March 2006

Published In

Volume / Issue

  • 38 / 3

Start / End Page

  • 356 - 362

PubMed ID

  • 16462742

Pubmed Central ID

  • 16462742

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng1744

Language

  • eng

Conference Location

  • United States