Ciliated epithelial cell lifespan in the mouse trachea and lung.

Journal Article (Journal Article)

The steady-state turnover of epithelial cells in the lung and trachea is highly relevant to investigators who are studying endogenous stem cells, manipulating gene expression in vivo, or using viral vectors for gene therapy. However, the average lifetime of different airway epithelial cell types has not previously been assessed using currently available genetic techniques. Here, we use Cre/loxP genetic technology to indelibly label a random fraction of ciliated cells throughout the airways of a cohort of mice and follow them in vivo for up to 18 mo. We demonstrate that ciliated airway epithelial cells are a terminally differentiated population. Moreover, their average half-life of 6 mo in the trachea and 17 mo in the lung is much longer than previously available estimates, with significant numbers of labeled cells still present after 18 mo.

Full Text

Duke Authors

Cited Authors

  • Rawlins, EL; Hogan, BLM

Published Date

  • July 2008

Published In

Volume / Issue

  • 295 / 1

Start / End Page

  • L231 - L234

PubMed ID

  • 18487354

Pubmed Central ID

  • PMC2494792

International Standard Serial Number (ISSN)

  • 1040-0605

Digital Object Identifier (DOI)

  • 10.1152/ajplung.90209.2008


  • eng

Conference Location

  • United States