Tissue interactions pattern the mesenchyme of the embryonic mouse lung.

Published

Journal Article

The mechanisms that control proliferation and differentiation of embryonic lung mesenchyme are largely unknown. We describe an explant system in which exogenous recombinant N-Sonic Hedgehog (N-Shh) protein sustains the survival and proliferation of lung mesenchyme in a dose-dependent manner. In addition, Shh upregulates several mesenchymal cell markers, including its target gene Patched (Ptc), intercellular signaling genes Bone Morphogenetic Protein-4 (Bmp4) and Noggin (Nog), and smooth muscle actin and myosin. In explants exposed to N-Shh in the medium, these products are upregulated throughout the mesenchyme, but not in the periphery. This exclusion zone correlates with the presence of an overlying mesothelial layer, which, as in vivo, expresses Fibroblast Growth Factor 9 (Fgf9). Recombinant Fgf9 protein inhibits the differentiation response of the mesenchyme to N-Shh, but does not affect proliferation. We propose a model for how factors made by two epithelial cell populations, the inner endoderm and the outer jacket of mesothelium, coordinately regulate the proliferation and differentiation of the lung mesoderm.

Full Text

Duke Authors

Cited Authors

  • Weaver, M; Batts, L; Hogan, BLM

Published Date

  • 2003-06-01

Published In

Volume / Issue

  • 258 / 1

Start / End Page

  • 169 - 184

PubMed ID

  • 12781691

Pubmed Central ID

  • 12781691

International Standard Serial Number (ISSN)

  • 0012-1606

Language

  • eng

Conference Location

  • United States