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Characterization of human Gadd45, a p53-regulated protein.

Publication ,  Journal Article
Carrier, F; Smith, ML; Bae, I; Kilpatrick, KE; Lansing, TJ; Chen, CY; Engelstein, M; Friend, SH; Henner, WD; Gilmer, TM
Published in: J Biol Chem
December 23, 1994

GADD45 (growth arrest and DNA damage) is a DNA-damage-inducible gene regulated in part by the tumor suppressor p53. A role in negative growth control has recently been suggested based on significant (more than 75%) reduction of colony formation following over expression of Gadd45. To better understand the role of Gadd45, we have developed specific rabbit and murine antibodies raised against the human recombinant protein. Using these antibodies, we have found that in ML-1 cells Gadd45 is predominantly a nuclear protein. MyD118, a protein induced by terminal differentiation sharing 57% homology with Gadd45, does not cross-react with any of the antibodies produced. As expected, the induction of Gadd45 protein by ionizing radiation (IR) was also found to be dependent on a wild type p53 phenotype. Interestingly, WI-L2-NS, a human lymphoid cell line, showed very high basal levels of Gadd45 mRNA and protein in addition to a high constitutive level of a mutated p53 protein. In this cell line, the high levels of GADD45 did not inhibit cellular growth in spite of the fact that no mutations were found in GADD45 sequence. These results indicate that some cell line(s) can tolerate high levels of Gadd45 and abrogate its growth suppression function.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

December 23, 1994

Volume

269

Issue

51

Start / End Page

32672 / 32677

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Spodoptera
  • Radiation, Ionizing
  • RNA, Messenger
  • Proteins
  • Protein Biosynthesis
  • Molecular Sequence Data
  • Intracellular Signaling Peptides and Proteins
  • Humans
  • Genes, p53
 

Citation

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Carrier, F., Smith, M. L., Bae, I., Kilpatrick, K. E., Lansing, T. J., Chen, C. Y., … Gilmer, T. M. (1994). Characterization of human Gadd45, a p53-regulated protein. J Biol Chem, 269(51), 32672–32677.
Carrier, F., M. L. Smith, I. Bae, K. E. Kilpatrick, T. J. Lansing, C. Y. Chen, M. Engelstein, S. H. Friend, W. D. Henner, and T. M. Gilmer. “Characterization of human Gadd45, a p53-regulated protein.J Biol Chem 269, no. 51 (December 23, 1994): 32672–77.
Carrier F, Smith ML, Bae I, Kilpatrick KE, Lansing TJ, Chen CY, et al. Characterization of human Gadd45, a p53-regulated protein. J Biol Chem. 1994 Dec 23;269(51):32672–7.
Carrier, F., et al. “Characterization of human Gadd45, a p53-regulated protein.J Biol Chem, vol. 269, no. 51, Dec. 1994, pp. 32672–77.
Carrier F, Smith ML, Bae I, Kilpatrick KE, Lansing TJ, Chen CY, Engelstein M, Friend SH, Henner WD, Gilmer TM. Characterization of human Gadd45, a p53-regulated protein. J Biol Chem. 1994 Dec 23;269(51):32672–32677.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

December 23, 1994

Volume

269

Issue

51

Start / End Page

32672 / 32677

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Spodoptera
  • Radiation, Ionizing
  • RNA, Messenger
  • Proteins
  • Protein Biosynthesis
  • Molecular Sequence Data
  • Intracellular Signaling Peptides and Proteins
  • Humans
  • Genes, p53